柿属植物超低温保存技术研究进展

Research Progress on Cryopreservation of Diospyros spp. Germplasm

我国是柿属植物(Diospyros spp.)的原产中心之一,柿属植物种质资源丰富,一般采用原位保存和田间保存两种方式,这两种保存方式存在着很大的风险。超低温保存是目前柿属植物种质资源长期稳定保存的理想方法之一。本文对其超低温保存的定义、方法及存在的问题和展望进行了论述,并对茎尖改良滴冻法进行了详细介绍。

China is one of the primary origins of Diospyros spp. and has abundant germplasms of D. spp. At present, most of these germplasms have been conserved in vivo and field gene banks. The two conventional methods have inherent risks. Cryopreservation is an ideal method for long-term conservation of Diospyros. germplasm. The paper reviewed research progresses on the cryopreservation of Diospyros, such as definition and methods of cryopreservation, some existing problems, especially cryopreservation of shoot tips using modified droplet-vitrification method was introduced.