通补奇经法复方对人子宫内膜基质细胞功能影响实验研究

Experimental Study on the Effect of Purgation-tonifying Method Compound Prescription on the Function of Human Endometrial Stromal Cells

目的 ：观察通补奇经法中药复方对人子宫内膜基质细胞（Human endometrial stroma cells,h ESCs）相关纤维连接蛋白（蛋白FN）、胰岛素样生长因子1（IGF-1）、催乳素（PRL）及纤溶酶原激活物抑制剂-1（PAI-1）表达的影响。方法 ：体外培养h ESCs,采用MTT法发现安全中药复方浓度为10^-4 mol/L,将实验分为溶剂对照组、甲孕酮（10^-7 mol/L）组、甲孕酮（10^-7 mol/L）＋中药（10^-4 mol/L）组、雌二醇（10^-8 mol/L）组、雌二醇（10^-8 mol/L）＋甲孕酮（10^-7 mol/L）组、雌二醇（10^-8 mol/L）＋中药试剂（10^-4 mol/L）组、雌二醇（10^-8 mol/L）＋甲孕酮（10^-7 mol/L）＋中药（10^-4 mol/L）组,采用ELISA方法检测各组子宫内膜基质细胞功能相关蛋白表达变化。结果 ：与溶剂对照组比较,各组FN、IGF-1、PRL蛋白表达量均显著升高（P〈0.05或P〈0.01）,而PAI-1表达显著减低,与甲孕酮组、雌二醇组分别比较,甲孕酮＋中药组、雌二醇＋甲孕酮组、雌二醇＋中药组、雌二醇＋甲孕酮＋中药组FN、IGF-1、PRL蛋白表达量均显著升高（P〈0.05或P〈0.01）,且甲孕酮＋中药组、雌二醇＋中药组、雌二醇＋甲孕酮＋中药组FN、IGF-1、PRL蛋白表达均分别高于对应的甲孕酮组、雌二醇组、雌二醇＋甲孕酮组（P〈0.05或P〈0.01）,而PAI-1呈现相反趋势。结论 ：通补奇经法中药复方可以促进人子宫内膜基质细胞相关蛋白的表达,其作用效果与甾体类激素相近。

Objective ：To observe the effects of traditional Chinese medicine purgation-tonifying method compound prescription on expression of fibronectin（FN）,insulin-like growth factor-1（IGF-1）,prolactin（PRL）and plasminogen activator inhibitor-1（PAI-1）in human endometrial stroma cells（h ESCs）. Methods ：h ESCs were cultured in vitro. Determined by MTT method to find safe drug concentrations of 10^-4 mol/L. Experiment can be divided into solvent control group,progestin（10^-7 mol/L）group,progestin（10^-7 mol/L）＋ Chinese medicine（10^-4 mol/L）group,estradiol（10^-8 mol/L）,estradiol（10^-8 mol/L）＋progestin（10^-7 mol/L）group,estradiol（10^-8 mol/L）＋ Chinese medicine（10^-4 mol/L）group,estradiol（10^-8 mol/L）＋progestin（10^-7 mol/L）＋ Chinese medicine（10^-4 mol/L）group and solvent control group. Using ELISA method to detect each group of endometrial stromal cells function related protein expression changes. Results ：Compared with solvent control group,each group FN,IGF-1,PRL,protein expression were significantly higher（P〈0.05 or P〈0.01）,while PAI-1 expression significantly reduced potential. Compared with the armour of progesterone,estradiol group respectively,progesterone＋Chinese medicine group,estradiol＋progesterone group,estradiol＋Chinese medicine group,estradiol＋ progesterone ＋ Chinese medicine group,FN,IGF-1,PRL protein expression were significantly higher（P〈0.05 or P〈0.01）,while PAI-1 presents the opposite trend. Conclusion ：Purgation-tonifying method compound prescription can promote the expression of basal cell related protein in human endometrium,and its effect is similar to that of steroid hormone.