微小RNA-130a和微小RNA-130b在肝硬化患者血清中的表达及其意义

Expression and significance of microRNA-130a and microRNA-130b in serum of patients with liver cirrhosis

目的研究肝硬化患者血清中微小RNA-130a(miR-130a)和miR-130b的表达及意义。方法收集15例肝硬化患者和15例健康志愿者的肘静脉血液,并分别设为试验组和对照组。用反转录聚合酶链式反应法检测血清中的miR-130a和miR-130b的表达情况。结果试验组和对照组的miR-130a相对表达量分别为(1.57±0.36)和(1.40±0.46),miR-130b相对表达量分别为(2.18±0.60)和(1.64±0.26),差异均有统计学意义(P<0.05或P<0.01)。结论 miR-130a和miR-130b在肝硬化患者血清中的表达量显著高于健康志愿者,其很有可能作为肝硬化早期诊断指标。

Objective To study the expression and significance of microRNA-130a（ miR-130a） and miR-130b in cirrhosis＇ serum.Methods The elbow vein blood of 15 cases of healthy subjects and 15 cases of patients with cirrhosis were collected and were divided into treatment group and control group respectively. Reverse transcription-polymerase chain reaction was used to detect the expression of miR-130a and miR-130b in serum. Results The relative expression of miR-130a in treatment group and control group were（ 1. 57 ± 0. 36）and（ 1. 40 ± 0. 46） and the relative expression of miR-130b in serum in treatment group and control group were（ 2. 18 ± 0. 60） and（ 1. 64 ± 0. 26）. The differences were statistically significant（ P 0. 05 or P 0. 01）. Conclusion The expression level of miR-130a and miR-130b in cirrhosis＇ serum is significantly higher than those in healthy person. It is likely to be used as a diagnostic marker for cirrhosis.