大黄和虎杖对血瘀症模型大鼠血流动力学和血脂的影响

Effects of Rhubarb,Polygonum cuspidatum on hemodynamics and blood lipid in rats with blood stasis syndrome

目的观察大黄和虎杖及其配伍对血瘀症模型大鼠血流动力学和血脂的影响。方法将40只SPF级雌性大鼠随机分为空白组、模型组、实验Ⅰ组、实验Ⅱ组和实验Ⅲ组,每组8只。实验Ⅰ、Ⅱ、Ⅲ组分别予以30 mg·kg^(-1)大黄、虎杖、大黄虎杖(1∶1),qd;空白组和模型组均按5 mL·kg^(-1)的剂量予以0.9%NaCl,qd。5组大鼠均连续灌胃10 d。第10天灌胃1 h后,除空白组外,其余各组均予以盐酸肾上腺素1 mg·kg^(-1),腹腔注射。24 h后,再次注射相同剂量的肾上腺素。急性血瘀模型制备1 h,将大鼠麻醉后腹主动脉采血。比较4组大鼠的血流动力学和血脂指标。结果空白组和实验Ⅲ组的全血黏度200/s分别为(3.26±1.33)和(4.51±1.15)mPa·s,50/s分别为(4.21±1.41)和(5.80±1.44)mPa·s,5/s分别为(9.75±2.97)和(13.27±3.14)mPa·s,1/s分别为(25.58±7.24)和(34.49±7.95)mPa·s,差异均有统计学意义(均P<0.05)。空白组、实验Ⅰ组、实验Ⅱ组红细胞聚集指数分别为(8.04±0.80),(7.39±0.31)和(7.18±0.62),空白组与实验Ⅰ组比较差异有统计学意义(P<0.05),空白组与实验Ⅱ组比较差异有统计学意义(P<0.01)。模型组和实验Ⅱ组的三酰甘油分别为(0.92±0.39)和(0.61±0.15)mmol·L^(-1),差异有统计学意义(P<0.05)。结论大黄、虎杖单用和大黄虎杖配伍合用均对血瘀证大鼠的血流动力学异常有较好的改善作用,但大黄虎杖1∶1配伍没有表现出明显的协同作用。虎杖能显著降低三酰甘油,而大黄单用没有表现明显降低血脂的作用,在降低血脂方面大黄和虎杖配伍合用也没有表现出协同作用。

Objective To explore the effect of Rhubarb and Polygonum cuspidatum and their combination on blood stasis model rats. Methods Forty female SPF rats were chosen and were randomly divided into blank,model,test Ⅰ,test Ⅱ,test Ⅲ groups,8 rats in each group. The rats of test Ⅰ,test Ⅱ,test Ⅲ groups were given 30 mg·kg^（-1） Rhubarb drug,Polygonum,Rhubarb Polygonum cuspidatum（ 1∶ 1）,qd; blank and model groups were given 0. 9% NaCl at 5 mL·kg^（-1） dose,qd. The rats were treated for consecutive 10 days. After lavage for 1 h,except the blank group,the rats in other groups were given adrennaline hydrochloride 1 mg·kg^（-1） by intraperitoneal injection. After 24 h,they were injected the same dose of adrenaline again. After the acute blood stasis model was prepared,the rats were anesthetized to collect blood from the abdominal aorta. After anticoagulation treatment,the hemorheology index and lipid index of all samples were tested by automatic blood rheology tester and automatic biochemical analyzer. Results The blood biscosity in blank and test Ⅲ groups were（ 3. 26 ± 1. 33） and（ 4. 51 ± 1. 15） mPa·s at 200/s,（ 4. 21 ± 1. 41） and（ 5. 80 ± 1. 44） mPa·s at 50/s,（ 9. 75 ± 2. 97） and（ 13. 27 ± 3. 14） mPa·s at 5/s,（ 25. 58 ± 7. 24） and（ 34. 49 ± 7. 95） mPa·s at 1/s,with significant difference（ all P 0. 05）. The erythrocyte aggregation index in the blank,test Ⅰ,test Ⅱ groups were（ 8. 04 ± 0. 80）,（ 7. 39 ± 0. 31） and（ 7. 18 ± 0. 62）,respectively. The difference between the blank and test I groups was statistically significant（ P 0. 05）. The difference between the blank and test Ⅱ groups were highly statistically significant（ P 0. 01）. Triglyceride in model,test Ⅱ groups were（ 0. 92 ± 0. 39） and（ 0. 61 ± 0. 15） mmol·L^（-1）,with significant difference（ P 0. 05）. Conclusion Rhubarb,Polygonum cuspidatum,and Rhubarb-Polygonum cuspidatum combination all had good effect on the rats with blood stasis syndrome,but Rhubarb-Polygonum cuspidatum combination（ 1 ∶ 1） did not show the obvious synergistic effect. Polygonum cuspidatum can significantly reduce triglycerides,while Rhubarb alone didn＇t show the role of significant reduction of blood lipids,and in reducing blood lipids,rhubarb and in this regard,Polygonum cuspidatum compatibility didn＇t show the synergistic effect.