长春瑞滨诱导人上皮性卵巢癌细胞系SKOV3凋亡及降低端粒酶活性

Vinorelbine induces apotosis and decreases telomerase activity in human epithelial ovarian cancer cells line SKOV3

目的探讨长春瑞滨对人上皮性卵巢癌细胞系SKOV3细胞凋亡、端粒酶活性及亚单位人端粒酶反转酶mRNA(hTERT mRNA)表达的影响。方法将不同浓度的长春瑞滨作用于SKOV3细胞后,用CCK-8法检测细胞增殖;流式细胞计量术检测细胞凋亡;端粒重复序列扩增酶联免疫吸附试验(TRAP-ELISA)检测端粒酶活性;RT-PCR检测细胞中hTERT mRNA表达。结果长春瑞滨可抑制SKOV3细胞增殖,诱导细胞凋亡(P<0.01),降低SKOV3细胞中端粒酶活性及hTERT mRNA的表达(P<0.01),且存在浓度-时间依赖关系。结论检测端粒酶的活性及hTERT mRNA表达,可能有助于判定长春瑞滨诱导上皮性卵巢癌细胞凋亡的敏感性。

Objective To investigate the effect of different concentrations of vinorelbine on apoptosis,telomerase activity and expression of human telomerase-reverse transcriptase gene（ hTERT） in human epithelial ovarian cancer cells SKOV3. Methods Ovarian cancer cells SKOV3 were treated with vinorelbine under different concentrations.The cell proliferation was measured by cell counting kit-8（ CCK-8） assay,and the cell apoptosis was detected by flow cytometry. The telomerase activity of SKOV3 cells was determined by TRAP-PAGE-silver staining; The mRNA expression of hTERT was performed by RT-PCR assay. Results Vinorelbine could significantly inhibited the proliferation of SKOV3 cells,induce cell apoptosis（ P〈0. 01）,reduce the telomerase activity and expression of hTERT mRNA（ P〈0. 01）,in dependent of a concentration-time manner. Conclusions The detection of telomerase activity and the mRNA expression of hTERT might be vital for predicting the prognosis of patients with epithelial ovarian cancer.