吉林省不同地区牛肠道病毒遗传变异分析

Genetic variation of BEV isolates in different areas of Jilin province

为了解近年来吉林省牛肠道病毒（bovine enteroviruses，BEV）感染流行情况及病毒株分子变异特征，本研究利用双抗体夹心ELISA方法，对采集于吉林省不同地区的326份疑似BEV感染的牛粪便样品进行检测，并对ELISA检测出的阳性样品进行病毒分离培养；同时对分离毒株5’UTR基因序列进行扩增、序列比对分析。结果表明，不同地区的牛群均存在程度不同的肠道病毒感染；BEV分离毒株的核苷酸序列与本实验室2012年分离的国内首株E种牛肠道病毒HY12基因序列的同源性为86．2％～96．9％。其中从公主岭地区分离的毒株GZL-6与HY12毒株差异性最大，其同源性只有86．2％；而与HY12同地区分离出的HY68毒株同源性只有86．3％。国内外现有BEV毒株序列遗传进化树分析结果显示，从吉林省新分离到11株BEV与HY12处在同一个分支。上述结果表明，新近分离的BEV毒株，无论源于HY12毒株的原地区还是其他不同地区，均有不同程度的核苷酸序列差异。本研究为BEV感染的诊断、防治及肠道病毒进化变异研究打下基础。

In order to investigate the prevalence of evolutionary characteristics of BEV isolates in Jilin bovine enterovirus infection and the molecular Province,326 suspected BEV samples were collected from different regions of Jilin Province. The positive samples detected by sandwich ELISA were processed for virus isolation. 5＇UTR sequences were amplified by RT-PCR, sequenced and aligned with known BEV strains in the GenBank for phylogenetie analysis by neighbor-joining methods. The results showed that BEV infections were widely detected from different regions. The sequences identities for the newly isolated virus were 86.2%-96.9% with HY12 virus. BEV GZL- 6 strain isolated from Gongzhuling region had the lowest sequence identity with HY12, and followed by HY68 strain isolated from the same area as HY12 with a sequence identitity of 86.3% with HY12. These newly isolated enterovirus srains were clustered to the same subgenotype as HY12 in the clade for enteroviruses E. The results in this study will provided a basis for the diagnosis and prevention of BEV infection,and study on enterovirus evolution.