氮掺杂碳量子点的制备及中药黄酮分析

Preparation of Nitrogen Doped Carbon Quantum Dots and Flavonoids Analysis in Chinese Herbal Medicine

以柠檬酸为主碳源加入乙二胺进行修饰,采用高温热解法一步合成氮掺杂的荧光碳量子点。该碳点的最佳激发波长为360 nm,发射波长为448 nm。该碳量子点在室温下荧光强度较为稳定。芸香叶苷对这些荧光碳点具有选择性猝灭的效果,这种现象可以用于不同领域黄酮的检测。荧光碳点的荧光强度随着芸香叶苷浓度的增加而逐渐衰减。该方法对芸香叶苷测定的线性范围为1.0×10^(-6)~8.0×10^(-5)mol/L。结果应用于中草药苍术、忍冬藤中黄酮含量测定,回收率在95.9%~101.8%的范围内,RSD分别为2.49%和1.96%。

Citric acid was used to the main carbon source with ethylenediamineas a modifier, nitrogen - doped fluorescent carbon quantum dotswas synthesized by high -temperature pyrolysis method. The optimal excitation and the emission wavelength of the carbon dots are 360 nm and 448 nm. Thecarbon quantum dots at room temperature can produce stable fluorescence intensity,rutin has the selective quenching effect on these fluorescent carbon quantum dots, which can be used for the detection of flavonoids in different fields. The fluorescent intensity of carbon quantum dots gradually decayed with the increase of rutin concentration. The linear range of rutin was 1.0 x 10-6 _ 8.0 x 10-s mol/L with this method. The results were applied to the determination of flavonoids in Chinese Herba of ArtemisiaeJaponicus and Houttuyniaeordata,and the recoveries ranged was from 95.9% to 101. 8% with RSD of 2.49% and 1.96% ,respectively.