HPLC-ELSD测定安神宝颗粒中3种成分

Determination of 3 ingredients in Anshenbao granules by HPLC-ELSD

为完善安神宝颗粒的质量控制标准,采用高效液相色谱法同时测定安神宝颗粒中酸枣仁皂A、东莨菪内酯、槲皮素3种有效成分,采用Agilent Zorbox SB C18色谱柱(4.6mm×150mm,5μm)及蒸发光散射检测器,柱温35℃,流动相为甲醇-0.5%的冰醋酸水溶液,梯度洗脱,流速为1.0mL/min,进样量10μL。结果表明,在该色谱条件下,3种有效成分均完全分离,峰面积对数与进样量对数呈良好的线性关系(r=0.995 6~0.998 0);精密度、重复性、稳定性试验结果符合要求,各被测组分的平均回收率在94.74%~96.08%之间。液相色谱法操作简单,准确度、灵敏度高,可用于检测安神宝颗粒中的有效成分,对完善安神宝颗粒质量标准,提升药品的质量控制水平具有实际意义。

In order to improve the quality control standard of Anshenbao granules,a high performance liquid chromatography(HPLC)method is developed for the determination of three active ingredients including Jujuboside A,Scopoletin and Quercetin in Anshenbao granules.The analysis is performed on a Agilent Zorbox SB C18 column(4.6 mm×150 mm,5μm)with a gradient mobile phase of methanol-0.5% glacial acetic acid at a flow rate of 1.0 mL/min.The evaporative light scattering detector(ELSD)is used,the column temperature is 35 ℃,and the injection is 10μL.The three active ingredients are separated completely under the given chromatogram system with a good linearity(r=0.995 6~0.998 0)of peak area and the injection.The results of precision,repeatability and stability experiments can meet the requirement,and the mean recoveries of each active ingredients are all in the range of 94.74%~96.08%.The analytical method is simple,accurate and sensitive,and it can be used for determination of active ingredients in Anshenbao granules.It is of practical significance to improve the quality standard of Anshenbao and improve the quality control of medicine.