NGF、ASIC3与实验性大鼠Ⅲ型前列腺炎骨盆疼痛的相关性研究

Expression of Nerve Growth Factor and Type 3 of Acid Sensitive Ion Channels in Rat Model of Type Ⅲ Prostatitis

目的探讨神经生长因子(NGF)、三型酸敏感离子通道(ASIC3)在实验性大鼠Ⅲ型前列腺炎的前列腺组织中表达的意义。方法选取性成熟雄性SD大鼠30只,分为对照组(0.9%NaCl于盆腔区域皮下、双侧肩胛皮下多点注射)、Ⅲ型前列腺炎模型组[实验性自发免疫性前列腺炎(EAP)组,完全弗氏佐剂与前列腺组织混悬液于盆腔区域皮下、双侧肩胛皮下多点注射],每组15只。建立EAP模型过程中,于建模第0、5、10、20、30、40天予Von-Frey测痛纤维进行骨盆区域痛觉测试。建模成功后取大鼠的前列腺组织,进行HE染色观察病理学改变;通过免疫组化和Western blot方法检测NGF及ASIC3的蛋白表达。结果 Von-Frey测痛结果显示:EAP组大鼠骨盆疼痛表现较对照组明显。HE染色结果显示:EAP组大鼠前列腺间质内淋巴细胞和中性粒细胞浸润,腺体周围充血;对照组未见明显炎症细胞浸润。免疫组化染色和Western blot结果显示:EAP组大鼠的前列腺组织中,NGF及ASIC3蛋白表达(主要表达于细胞核与细胞浆中)较对照组增加(P<0.01)。结论EAP大鼠前列腺组织中NGF及ASIC3表达增加,NGF及ASIC3可能是导致Ⅲ型前列腺炎骨盆区域疼痛的重要介质,有望作为干预及治疗Ⅲ型前列腺炎的靶向指标。

Objective To investigate the expressions of nerve growth factor（NGF）and acid-sensing ion channel 3 （ASIC3） in prostatic tissue of experimental rats with type Ⅲ prostatitis. Methods Thirty SD rats were randomly allocated into control group and experimental group. The rats in control group were subjected to pelvic and bilateral scapular subcutaneous injections of 0.9% sodium chloride, while the rats in experimental group were given pelvic and bilateral scapular subcutaneous injections of mixed suspension of complete Freund＇ s adjuvant and prostatic tissue to induce autoimmune prostatitis （EAP）. Tactile allodynia was quantified using Von-Frey as a measure of pelvic pain behavior. This measurement was performed on 0th , 5th, 10th , 20th , 30th and 40th day in the two groups. After that, the prostate samples were collected and processed for HE staining, while the expressions of NGF and ASIC3 were measured by immunohistochemistry and Western blot. Results Von-Frey filaments measurement showed that pelvis pain in EAP group was significantly more obvious than that in control group. HE staining found lymphocytes and neutrophils infiltrated in the prostate of EAP rats, hut no inflammatory cells in the prostate of control group rats. The expressions of NGF and ASIC3 were significantly increased in EAP group when compared with control group （P〈0.01）. Conclusion The expressions of NGF and ASIC3 in the prostate with EAP were significantly increased, which may be the important mediators of chronic pelvic pain.