AXIN和MACC1蛋白在胃癌组织中的表达及其临床意义

Expression of AXIN and MACC1 in Gastric Carcinoma and Its Clinical Significance

目的检测胃癌组织中轴蛋白(AXIN)和结肠癌转移相关基因1(MACC1)蛋白的表达,分析其相互间及与胃癌临床病理参数以及预后的关系。方法采用免疫组织化学ElivisionTMplus法检测100例胃癌组织(胃癌组)和60例正常胃黏膜组织(对照组,胃癌患者手术切除标本的切缘组织,距离胃癌肿块>5.0cm,确认无癌细胞)中AXIN和MACC1蛋白的表达。结果在胃癌组织中AXIN和MACC1蛋白阳性表达率分别为37.0%和58.0%,对照组中AXIN和MACC1蛋白表达的阳性率分别为83.3%和6.7%,其表达差异均有统计学意义(P<0.05)。AXIN和MACC1在胃癌组织中的表达均与胃癌的分化程度、浸润深度、淋巴结是否转移以及不同Duke分期等有关(P<0.05),且AXIN蛋白表达的阳性率与MACC1蛋白表达的阳性率之间呈负相关(r=-0.355,P<0.001)。Kaplan-Meier法生存分析显示,AXIN和MACC1蛋白表达阳性组与阴性组之间的5年生存率差异均有统计学意义(P均<0.001)。Cox多因素分析显示,AXIN的阴性表达和MACC1的阳性表达及Duke分期(Ⅲ~Ⅳ期)是影响胃癌患者预后的独立危险因素。结论 AXIN和MACC1蛋白的表达与胃癌患者的预后有关,参与了胃癌的侵袭及转移。

Objective To investigate the expression of axis inhibition protein （AXIN） and metastasis-associated in colon cancer-1 （MACC1） in gastric carcinoma and their relationship to the clinicopathologic characteristics. Methods Expressions of AXIN and MACC1 proteins were examined by immunohistochemistry containing 100 specimens of gastric tissues （gastric carcinoma group） and 60 specimens of normal gastric mucosa tissues （control group, the nearby tissues of the excised specimen of gastric cancer patients, from the tumor of the gastric cancer 〉5.0 cm, and confirm that there were no cancer cells）. Results The positive rates of AXIN and MACC1 proteins in gastric carcinoma and the control tissues were 37. 0% vs. 83. 3% and 58. 0% vs. 6. 7%, respectively. The difference were significant between the two groups （both P〈0.05）. The expressions of AXIN and MACC1 proteins were significantly related with grades of tumor, depth of invasion, lymph node metastasis, and Duke stages （P%0. 05）. Spearman analysis showed that there was a negative relationship between the AXIN expression and MACC1 expression （r=-0. 355, P%0.05）. Kaplan-Meier survival analysis and log-rank single factor analysis showed that AXIN and MACC1 protein expressions were related to the 5-year survival rate of patients （both P〈0.05）. Cox regression analysis showed that the positive expression of AXIN and the negative expression MACC1 protein, and Duke stages （ III-IV ） were the independent prognostic factors of gastric carcinoma. Conclusion The expressions of AXIN and MACC1 proteins are related to the prognosis of gastric carcinoma patients, and are involved in the invasion and metastasis of gastric carcinoma.