急性心肌梗死患者血清MCP-1水平及其与MCP-1 2518G/A多态性的关系

The relationship between serum MCP-1 level and MCP-1 2518G/A polymorphism in patients with acute myocardial infarction

目的探索中国苏北涟水县汉族人群急性心肌梗死(AMI)患者血清单核细胞趋化蛋白1(MCP-1)水平及其与MCP-1 2518G/A多态性的关系。方法 73例无冠状动脉病变者(对照组)和94例AMI患者(AMI组)的血清MCP-1水平采用ELISA检测,MCP-1 2518G/A多态性采用PCR-RFLP、DNA测序技术方法检测。结果血清MCP-1水平(以M/IQR表示)在AMI组为186.24/285.15 ng/L,显著高于对照组的100.71/134.02 ng/L(P=0.001)。多元线性回归分析显示,血清MCP-1水平与男性负相关,与高血压、糖尿病、吸烟正相关,与老龄和血脂异常无明显相关。经校正高血压、糖尿病、年龄、性别、吸烟和血脂水平等因素后,≥75%分位数的血清MCP-1水平与AMI的发病风险呈正相关(OR=2.764,95%CI为1.061~7.204,P=0.037)。MCP-1 2518G/A基因型分布和等位基因频率在AMI组与对照组之间无显著差异(P>0.05),血清MCP-1水平在对照组、AMI组各基因型间无显著差异(P>0.05)。结论中国苏北涟水县汉族人群的AMI患者血清MCP-1水平显著高于对照者;血清MCP-1水平受性别、高血压、糖尿病、吸烟影响,但≥75%分位数的血清MCP-1水平是AMI的独立预测因素;MCP-1 2518G/A多态性不增加AMI的易感性,也不影响MCP-1血清水平。

Aim To explore the relationship between serum monocyte chemoattractant protein-1（MCP-1）level and MCP-1 2518G/A polymorphism in patients with acute myocardial infarction（AMI）in Chinese Han population in Lianshui County of Northern Jiangsu.Methods Serum MCP-1 level was measured by ELISA,and the MCP-1 2518 G/A polymorphism was genotyped by PCR-RFLP in 94 patients with AMI and 73 control subjects.Results Serum MCP-1level（expressed in M/IQR）was significantly higher in AMI group（186.24/285.15 ng/L）than that in control group（100.71/134.02 ng/L,P=0.001）.Multiple linear regression analysis revealed that the serum MCP-1 level was negatively correlated with male,positively correlated with smoking,history of diabetes mellitus and hypertension,but unrelated with old age and dislipidemia in AMI group.After adjustment for hypertension,diabetes mellitus,age,gender,dislipidemia,and smoking,serum MCP-1 level of higher than 75%quantile was positively correlated with the risk of AMI（OR=2.764,95%CI was 1.061-7.204,P=0.037）.There was no significant difference in genotype distribution and allele frequency of MCP-1 2518G/A between AMI group and control group（P〉0.05）.There was no significant difference in serum MCP-1 level among the genotypes of the control group and the AMI group（P〉0.05）.ConclusionSerum MCP-1 level was significantly higher in AMI group than that in control group in Chinese Han population in Lianshui County of Northern Jiangsu.Serum MCP-1 level was affected by sex,hypertension,diabetes,and smoking,but serum MCP-1level of higher than 75%quantile was an independent predictor of AMI.MCP-1 2518G/A polymorphism did not increase the susceptibility of AMI,nor affect the serum level of MCP-1.