pH法防止转基因蓝藻向环境释放

pH method to prevent the release of transgenic Anabaena sp.into environment

[目的]防止转基因蓝藻(Anabaena sp.PPC 7120)在制备对虾抗白斑综合征病毒口服药过程中向环境释放,保证制药安全性。[方法]用不同pH处理转基因蓝藻,并在不同时间、温度和光照下观察效果,并用响应面法优化处理条件。[结果]最佳处理条件为:pH≤2.5、t≥50 min、T≥30℃或pH≥12、t≥120 min、T≥30℃,杀藻率为100%。两个优化组合分别为:pH 2.12、温度29.96℃、时间33.9 min或pH 12.33、温度31.75℃、时间96.8 min。pH处理后的转基因蓝藻在平板上不能再继续生长。[结论]pH法对转基因蓝藻的杀藻率可达100%,有效防止了转基因蓝藻在制备对虾口服剂过程中发生环境释放。

[Obiective]The release of Anabaena sp.PPC 7120 into environment during the preparation of the oral shrimp drug would be stopped,and pharmaceutical safety would be ensured.[Methods] The transgenic Anabaena sp.was treated with different pH and observed at different times,temperatures and illuminations.The response surface method was used to optimize the treatment conditions.[Results]The optimum treatment conditions were as follows： pH≤2.5,t≥50 min,T≥30 ℃ or pH≥12,t≥120 min,T ≥ 30 ℃,and the killed cell rate is up to 100%.The two optimal combinations were as follows： pH 2.12,29.96 ℃,33.9 min or pH 12.33,31.75 ℃,96.8 min.After the treatment with pH,the transgenic Anabaena sp.was not able to continue to grow on the plate completely.[Conclusion] The killed cell rate of the pH method up to 100% which can effectively prevent the release into environment of transgenic Anabaena sp.during the preparation of the oral shrimp drug.