小鼠骨髓基质细胞的辐射敏感性及某些药物对其作用的观察

Radiosensitivity of Marrow Stromal Cells and the Effect of Some Radioprotective Agents

本实验证实,小鼠股骨骨髓(BM)基质由成纤维细胞、网状细胞、吞噬细胞及脂肪细胞构成。在本外,由2根股骨的BM细胞所形成的贴壁细胞层对造血多能干细胞CFU-S支持功能最好。BM基质细胞为辐射敏感性低于造血干细胞;AET和兜衣多糖对BM基质细胞及其支持造血干细胞的功能有明显的辐射防护作用。

The results showed that marrow stromal cells include fibroblasts, recticular cells, macrophages and adipocytes.The capability of the adherent layer derived from marrow cells of 2 mouse femurs to support hematopoietic stem cells was stronger than those of layers derived from 0.5 or 1 mouse femurs.The radiosensitivity of bone marrow stromal cells was lower than that of hematopoietic stem cells.The radioprotective effect of AET and PLP (polysaccharide of Lobaria pulmonaria Hoffm) on the bone marrow stromal cells and their capability to support hematopoietic stem cells was clearly demonstrated.