应用PCR-DGGE技术分析稻谷储藏过程中的真菌多样性

Fungal diversity in paddy storage based on PCR-DGGE

采用传统分离培养和基于18SrDNA作为分子标记的变性梯度凝胶电泳(PCR-DGGE)的方法对新收获稻谷储藏期间的真菌多样性进行分析。结果表明,运用该法可以快速检出传统培养法不能检出的Rhodotorula mucilaginosa、Saccharomyces cerevisiae、Cryptococcus aureus、Phoma sp、Chaetosartorya cremea、Trichosporon caseorum等霉菌,且该法操作简便,为粮食霉菌的检测提供了一个高效、便捷的方法,同时极大地补充了储粮真菌的真菌库。

PCR-DGGE was used to analyze the diversity of mold during storage. The results showed that the Rhodotorula mucilaginosa , Saccharomyces cerevisiae , Cryptococcus aureus , Phoma sp, Chaetosartorya cremea and Trichosporon caseorum could not be detected by the traditional culture method, but PCR-DGGE was a rapid way and very easy to operate. It is very ef- ficient and convenient, at the same time, this method could greatly improve the fungi library of paddy storage.