CdTe/CdS量子点荧光猝灭法测定安奈格列汀含量

Determination of anagliptin with fluorescence quenching based on CdTe/CdS quantum dots

以Te粉、NaBH_4、Cd(NO_3)_2、硫代乙酰胺为反应物,合成了巯基乙酸稳定的CdTe/CdS量子点(QDs)。该量子点在345 nm激发时发出570 nm左右的荧光,而降糖药安奈格列汀对该处荧光有猝灭作用。研究发现在pH 7.0的介质中荧光猝灭效果最佳,并且当安奈格列汀浓度在0.5~10.0μg·mL^(-1)时荧光猝灭程度与其浓度成线性,线性方程为ΔF/F_0=0.0102+0.0312c(μg·mL^(-1)),r=0.997,检出限为0.15μg·mL^(-1),据此提出了测定安奈格列汀含量的荧光分析方法。使用本法成功测定了安奈格列汀药片、人血清中安奈格列汀含量。

CdTe/CdS quantum dots were prepared by the reaction of Te, NaBH4, Cd（NO3） 2 and Thioacetamide in water with mer- captoacetic acid as stabilizer. The quantum dots have strong fluorescence in 570 nm when excited at 345 nm,and hypoglycemic drug anagliptin would quench its fluorescence light. I t was found that the best fluorescence quenching effect was obtained in the media of pH 7. 0, and a linear quenching relationship was observed between fluorescence quench value and anagliptin concentration of 0. 5 - 10. 0 μg·mL^-1. The linear equation was AF/F0 =0. 0102＋0. 0312 c（ μg·mL^-1 ）and the detection limit was 0. 15 μg·mL^-1（ r = 0. 997）. Therefore a new fluorescence method for determining anagliptin was presented. The studied method was successfully used to determine anagliptin in anagliptin tablet and human serum samples.