骨肉瘤组织中NOV基因表达量检测及与细胞增殖、迁移的相关性研究

NOV gene expression in osteosarcoma tissue and its correlation with cell proliferation and migration

目的:探讨骨肉瘤组织中肾母细胞瘤过表达基因(NOV)表达量检测及与细胞增殖、迁移的相关性。方法:2014年5月~2017年8月在孝感市中心医院接受手术治疗的骨肉瘤患者的病灶组织及瘤旁正常组织各97例,分别作为骨肉瘤组、正常对照组。采用荧光定量PCR检测不同组织标本中NOV基因、增殖相关基因、侵袭相关基因的表达量,采用Pearson检验评估骨肉瘤组织中NOV基因表达量及其与细胞增殖、迁移的相关性。结果:骨肉瘤组中NOV mRNA的表达量显著低于正常对照组(P<0.05);骨肉瘤组中KISS-1、TAp73 mRNA的表达量低于正常对照组(P<0.05),VCP、FoxM1、RIPK4、STIM1mRNA的表达量高于正常对照组(P<0.05);骨肉瘤组中DLX1、Sema6A mRNA的表达量低于正常对照组(P<0.05),EFEMP1、GPR56、SOX5、CRYAB mRNA的表达量高于正常对照组(P<0.05)。Pearson检验发现,骨肉瘤组织中NOV基因表达量与细胞增殖基因、侵袭基因的表达量直接相关。结论:骨肉瘤组织中NOV基因表达量低于瘤旁正常组织,且具体表达量与肿瘤细胞增殖、侵袭活性直接相关,可作为骨肉瘤恶性程度判断的可靠指标。

Objective:To investigate the NOV gene expression in osteosarcoma tissue and its correlation with cell proliferation and migration.Methods:97 cases each of osteosarcoma tissue and adjacent normal tissue from patients with osteosarcoma who underwent surgical treatment in the Central Hospital of Xiaogan between May 2014 and August 2017 were selected as the osteosarcoma group and the normal control group respectively.Fluorescence quantitative PCR was used to detect the expression of NOV gene,proliferation-related genes and invasion-related genes in different tissue samples,and Pearson test was used to evaluate the NOV gene expression in osteosarcoma tissue and its correlation with cell proliferation and migration.Results:NOV mRNA expression in osteosarcoma group was significantly lower than that in normal control group;KISS-1 and TAp73 mRNA expresion in osteosarcoma group were lower than those in normal control group whereas VCP,FoxM1,RIPK4 and STIM1 mRNA expression were higher than those in normal control group;DLX1 and Sema6 A mRNA expression inosteosarcoma group were lower than those in normal control group whereas EFEMP1,GPR56,SOX5 and CRYAB mRNA expression were higher than those in normal control group.Pearson test showed that the NOV gene expression in osteosarcoma was directly correlated with the expression of cell proliferation genes and invasion genes.Conclusion:NOV gene expression in osteosarcoma tissue is lower than that in adjacent normal tissue,and the specific expression is directly correlated to tumor cell proliferation and invasion activity,and can be as a reliable index to judge the malignant degree of osteosarcoma.