栅藻全转录组测序与类胡萝卜素合成途径相关基因分析

RNA-seq based transcriptome analysis and the biosynthesis pathway of carotenoid in Desmodesmus sp.

栅藻(Desmodesmus sp.)是一种能产生重要次生代谢产物类胡萝卜素的海洋绿藻,为了深入了解栅藻类胡萝卜素代谢途径及关键酶基因,本研究通过Illumina高通量测序平台对栅藻进行转录组测序.利用Trinity软件对转录组数据进行从头组装,共获得37 634个Unigenes,经过与Nt、Nr、Swiss、Prot、KEGG、COG、GO数据库比对,共有23 235个Unigenes获得注释.GO分类中(level 2)注释最多的分别是代谢过程(metabolic process),细胞组分(cell part)和催化活性(catalytic activity),与KEGG数据库比对发现14 123个Unigenes与128条代谢途径相对应.根据组装和注释的转录组结果,鉴定了栅藻中与类胡萝卜素生物合成途径相关的基因,构建了栅藻类胡萝卜素代谢途径,获得的栅藻转录组数据有利于进一步研究类胡萝卜素代谢途径中相关基因的功能及调控.

Desmodesmus sp. is an important green algae,which has the ability to produce interesting secondary metabolites,such as biodiesel and carotenoid. With the purpose to investigate the carotenoid biosynthesis pathway in Desmodesmus sp.,RNA-seq was employed to analyze the gene involved in the biosynthesis pathways. After sequencing and de novo assembly,a total of 37 634 Unigenes were obtained,23 235 out of 37 634 Unigenes were annotated in at least one database,the most prominent GO categories( level 2) were metabolic process,cell part,and catalytic activity,respectively. 14 123 Unigenes were mapped to 128 biological pathways in the KEGG database.On the basis of the GO terms and KEGG annotation,candidate genes that encode enzymes involved in carotenoid biosynthetic were annotated,and the main carotenoid biosynthetic pathway was elucidated. The transcriptome provides a comprehensive sequence resource for the study of gene function and regulation of carotenoid biosynthesis pathways in this species.