辛伐他汀诱导胃癌BGC-823细胞凋亡的效应研究

Effect of simvastatin on apoptosis of gastric cancer BGC-823 cells

目的探讨辛伐他汀诱导胃癌BGC-823细胞凋亡的效应。方法将辛伐他汀终浓度为5.00μmol/L、2.50μmol/L、1.25μmol/L、0.63μmol/L的4个反应体系分别定义为A组、B组、C组、D组,采用CCK-8细胞增殖法检测A^D组的胃癌BGC-823细胞生长抑制率,分析其药物半数抑制浓度(IC50)。此外,再根据IC50设计3个辛伐他汀反应体系,辛伐他汀终浓度分别为2.00μmol/L、1.00μmol/L、0.00μmol/L,分别定义为E组、F组、G组,采用流式细胞法分析这E^F组的胃癌BGC-823细胞凋亡率。结果⑴A组、B组、C组、D组胃癌BGC-823细胞生长抑制率比较差异有统计学意义(P<0.01),A组生长抑制率显著高于B组、C组、D组(P<0.01),B组生长抑制率显著高于C组、D组(P<0.01),C组生长抑制率显著D组(P<0.01)。辛伐他汀对胃癌BGC-823细胞的IC50为1.15μmol/L。⑵E组、F组、G组胃癌BGC-823细胞凋亡率比较差异有统计学意义(P<0.01),E组凋亡率显著高于F组、G组(P<0.01),F组凋亡率显著高于G组(P<0.01)。结论辛伐他汀可显著抑制胃癌BGC-823细胞生长并诱导其凋亡。

Objective To investigate the effect of simvastatin on apoptosis of gastric cancer BGC-823 cells. Methods The 4 reaction systems with the final concentration of simvastatin at 5.00μmol/L,2.50μmol/L,1.25μmol/L and 0.63μmol/L were defined as group A,group B,group C and group D,respectively,and the CCK-8 cell proliferation assay was used to detect the growth inhibitory rate of gastric cancer BGC-823 cells in group A^D,and the median inhibitory concentration(IC50) was analyzed. Moreover,3 simvastatin reaction systems,with the final concentration of simvastatin at 2.00μmol/L,1.00μmol/L and 0.00μmol/L,respectively,were designed according to the IC50,which were defined as group E,group F and group G,respectively,then the apoptosis rate of gastric carcinoma BGC-823 cells in group E^F were detected by flow cytometry method. Results ⑴There were obvious differences in the growth inhibitory rate of gastric cancer BGC-823 cells among group A,group B,group C and group D(P<0.01),and the growth inhibitory rate in group A was obviously higher than that in group B,group C and group D(P<0.01),and the growth inhibitory rate in group B was obviously higher than that in group C and group D(P<0.01),and the growth inhibitory rate in group C was obviously higher than that in group D(P <0.01). The IC50 of simvastatin in gastric cancer BGC-823 cells was 1.15μmol/L. ⑵There were obvious differences in the apoptosis rate of gastric carcinoma BGC-823 cells among group E,group F and group G(P<0.01),and the apoptosis rate in group E was obviously higher than that in group F and group G(P<0.01),and the apoptosis rate in group F was obviously higher than that in group G(P<0.01). Conclusion The simvastatin is able to significantly inhibit the gastric cancer BGC-823 cells proliferation and induce apoptosis.