用免疫荧光McAb对286株脊髓灰质炎病毒标本进行定型诊断

The Application of Immunofluorescence and Neutralization McAb Tests to Typing Diagnosis of 286 Polio Strains

用PcAb血清NT与F-McAb IF对286株Polio Ⅰ、Ⅱ和Ⅲ型病毒进行定型,结果两者的符合率Ⅰ型为100％,Ⅱ型和Ⅲ型均为99％。Ⅰ型与Ⅱ、Ⅲ型符合率的微小差异,可能是实验技术上的误差所致,也可能是McAb本身识别抗原表位的局限性所致。提示要得到真正理想的型特异McAb,必须用大量有代表性的毒株进行交叉试验。本试验证明,F-McAb IF可以代替PcAb参考血清NT作为Polio病毒的定型方法。IF比NT简单、快速、特异和敏感,是组装试剂盒的理想材料。

The ssrum neutralization test is a classical method used for typing poliovi rus. Since 1987, some type-specific non-neutralization McAb's have been obtained in our laboratory, and were used in indirect imtnunofluorescence straining tes s to detect serotypes Ⅰ,Ⅱ and Ⅲ. A total of 286 poliovirus strains were typed by the indirect immunofluorescence test (IF)and neutralization test (NT). The results demonstrated that both tests were in agreement, suggesting that McAb IF could replace NT as an alternative typing diagnostic method, moreover, IF was easier to perform than NT. It is economical and rapid(the results can be read within 1-2 days as compared to 7 days for NT). The reagent is very stable and can be stored for two years at 4℃ or for two months at 37℃.