注射用丹参多酚酸与阿司匹林联合应用对抗凝效果和出血风险的影响

Anticoagulation Effect and Bleeding Risk of Combination Therapy with SAFI and Aspirin

目的研究注射用丹参多酚酸(SAFI)与阿司匹林联合应用抑制血小板聚集作用和对凝血功能的影响,为临床中西药合理联合用药提供实验依据。方法利用断尾法评价联合用药对小鼠尾部出血时间的影响;采用酶标仪法测定药物对二磷酸腺苷(ADP)、花生四烯酸(AA)、胶原(collagen)、凝血酶(thrombin)4种诱导剂作用下正常大鼠的血小板最大聚集率;测定凝血4项来评价联合用药对小鼠体内凝血功能的影响。结果出血时间:联合用药后SAFI对阿司匹林作用的断尾小鼠出血时间无明显影响。抗血小板聚集:与对照组相比,SAFI、阿司匹林均可显著抑制ADP、胶原、AA和凝血酶诱导的血小板聚集(P<0.01);联合用药后SAFI可进一步增强阿司匹林抗ADP、胶原、AA和凝血酶诱导血小板聚集的效果(P<0.01,P<0.05)。凝血功能:联合用药后SAFI降低阿司匹林作用的凝血酶时间。结论 SAFI与阿司匹林联合应用可增强抗凝效果,并且不增加阿司匹林的出血风险。

OBJECTIVE To explore the curative effect of combination therapy with salvianolate injection （SAFI） and aspirin on anti-platelet aggregation and blood coagulation function, provide the experimental evidence for clinical consequence use of combination therapy with traditional Chinese medicine and western medicine. METHODS The bleeding time was measured by cutting tail evaluating of combination of drugs ; platelet maximum aggregation rate induced by ADP, AA, collagen and thrombin in rats was determined by absorbance method using a microplate reader; blood coagulation of the four indicators were measured by the semiautomatic blood agglutination instrument. RESULTS SAFI had nearly no effects on aspirin group＇s bleeding time. The effect on blood platelets aggregation： compared with control group, SAFI group and aspirin group could significantly reduce platelet aggregation induced by ADP, AA, collagen and thrombin in normal rats（P 〈0. 01 ）. Combinative group could significantly increase aspirin group＇s anti-platelet aggregation induced by ADP, AA, collagen and thrombin in normal rats（ P 〈 0. 01 ,P 〈 0. 05 ）. Coagulation function ： SAFI can reduced aspi- rin group＇s TT after the combination therapy. CONCLUSION SAFI can increase anticoagulation effect of aspirin, and have no effect on aspirin＇s bleeding risk.