飞龙掌血醇提物的抗炎镇痛作用

Anti-inflammatory and analgesic activities of ethanol extract of Toddalia asiatica

目的研究飞龙掌血醇提物的抗炎镇痛作用及其机制。方法大鼠用角叉菜胶致炎及福尔马林致痛,研究飞龙掌血醇提物的镇痛作用;ELISA试剂盒检测角叉菜胶实验大鼠血清中β内啡肽(β-EP)、前列腺素E2(PGE2)、5-羟色胺(5-HT)的含有量,炎症大鼠皮肤组织中肿瘤坏死因子-α(TNF-α)及白细胞介素-1β(IL-1β)的含有量,以及福尔马林实验小鼠血清中白三烯B4(LTB4)的含有量;免疫组化法观察大鼠脊髓FOS及P物质(SP)蛋白表达。结果飞龙掌血醇提物能明显降低足趾肿胀率;福尔马林实验中,飞龙掌血醇提物能明显减少舔足累积时间,降低PGE2的含有量,高剂量组尤为明显;3个剂量组均能降低血清中5-HT的含有量,高剂量和低剂量效果显著,高剂量组还能升高血清中β-EP的含有量。各剂量组均能降低皮肤组织中TNF-α和IL-1β的含有量,中、高剂量组抑制FOS蛋白表达;高剂量组明显降低血清中LTB4的含有量。结论飞龙掌血醇提物具有抗炎镇痛作用,其作用机制可能是与通过增加血清中β-EP,降低PGE2、5-HT、LTB4含有量,降低皮肤组织中TNF-α、IL-1β含有量,以及下调脊髓SP及FOS蛋白表达有关。

AIM To study the anti-inflammatory and analgesic activities of ethanol extract of Toddalia asiatica Lam. and the mechanism. METHODS Both inflammatory rat model induced by carrageenan and pain model in- duced by formalin were applied to investigating the analgesic effect of extract of Toddalia asiatica Lain. ELISA kit was used to detect the contents of β-EP, 5-HT and PGE2 in serum of carrageenan-treated rats, contents of TNF-α and IL-1β in skin tissue of inflammatory rats, and content of LTB4 in serum of formalin-treated rats ; immunohisto- chemical method was used to observe the SP and FOS protein expressions in rat spinal cord. RESULTS The eth- anol extract of Toddalia asiatica Lam. could significantly reduce the rate of toe swelling. In the formalin test, the ethanol extract of Toddalia asiatica Lam. reduced not only the total licking time, but also the content of PGE2, es-pecially in the high dose group. And lowered serum 5-HT contents were observed in all the three dose groups, but a much better performance was found in both the high and low dose groups, and the high dose group＇ s capability in increasing serum β-EP content was also noticed. TNF-α and IL-1β contents in skin tissue were reduced in various dose groups. Middle and high dose groups inhibited FOS protein expression. And the content of LTB4 in serum was obviously decreased in the high dose group. CONCLUSION The anti-inflammatory and analgesic activities of ethanol extract of Toddalia asiatica Lam. may associate with its power in increasing β-EP in serum, decreasing PGE2,5-HT, LTB4 contents, reducing TNF-α, IL-1β contents in skin tissue, and lowering SP and FOS protein expressions in spinal cord.