HPLC法同时测定四磨汤口服液中5种成分

Simultaneous determination of five constituents in Simotang Oral Liquid by HPLC

目的建立HPLC法同时测定四磨汤口服液(木香、枳壳、槟榔等)中5种成分的含有量。方法该药物50%甲醇提取液的分析采用Agilent Zorbax C18色谱柱(4.6 mm×250 mm,5μm);流动相乙腈-0.1%甲酸,梯度洗脱;柱温35℃;检测波长283 nm;体积流量1.0 m L/min。结果辛弗林、去甲异波尔定、柚皮苷、橙皮苷、新橙皮苷分别在5.8~185.6μg/m L(r=0.999 9)、0.829~26.52μg/m L(r=1.000 0)、9.775~312.8μg/m L(r=1.000 0)、0.594~19μg/m L(r=0.999 5)、5.2~166.4μg/m L(r=1.000 0)范围内呈良好的线性关系,平均加样回收率分别为98.93%、98.95%、102.57%、99.67%、103.43%,RSD分别为1.85%、1.27%、0.52%、0.89%、0.43%。结论该方法简便、快速、准确、重复性好,可用于四磨汤口服液的质量控制。

AIM To establish an HPLC method for the simultaneous content determination of five constituents in Simotang Oral Liquid （ Aucklandiae Radix, Aurantii Fructus, Arecae Semen, etc. ）. METHODS The analysis of 50% methanol extract of this drug was performed on a 35 ℃ thermostatic Agilent Zorbax C18 column （4. 6 mm × 250 mm, 5 μm） , with the mobile phase comprising of acetonitrile-0. 1% formic acid flowing at 1.0 mL/min in a gradient elution manner, and the detection wavelength was set at 283 nm. RESULTS Synephrine, norisoboldihe, naringin, hesperidin and neohesperidin showed good linear relationships within the ranges of 5.8 - 185.6 μg/mL （ r = 0. 999 9 ）, 0. 829 - 26.52 μg/mL （ r = 1. 000 0）, 9.775 - 312. 8 μg/mL （ r = 1. 000 0 ）, 0.594- 19 μg/mL （r =0.999 5） and 5.2- 166.4 μg/mL （r = 1.000 0）, whose average recoveries were 98.93% , 98.95% , 102. 57%,99.67% and 103.43% with theRSDsofl. 85% , 1.27%,0.52%,0.89% and 0.43% , respectively. CONCLUSION This simple, accurate and reproducible method can be used for the rapid quality control of Simotang Oral Liquid.