铝碳酸镁对幽门螺杆菌的抑制作用及其机制

Mechanism underlying the inhibitory effects of Hydrotalcite on Helicobacter pylori

目的 探讨铝碳酸镁对H.pylori的抑制作用及其机制。 方法 采用哥伦比亚固体培养基和布氏肉汤液体培养基复苏、培养H.pylori国际标准菌株26695，并将其分为空白对照组、H.pylori损伤组和H.pylori联合铝碳酸镁组。用铝碳酸镁处理H.pylori菌液，观察H.pylori的活性变化，革兰染色方法观察H.pylori的形态变化。用考马斯亮蓝染色法检测H.pylori菌体蛋白质表达的改变情况。比较有无铝碳酸镁的情况下，H.pylori对胃黏膜上皮细胞GES-1凋亡的影响，透射电子显微镜和扫描电子显微镜下观察亚细胞结构及H.pylori对细胞的吸附能力。统计学处理采用t检验。 结果 铝碳酸镁可抑制H.pylori生长，但H.pylori形态未见明显改变，多种H.pylori菌体蛋白质表达受铝碳酸镁调控。未加铝碳酸镁时，H.pylori大量吸附在GES-1细胞表面，促进GES-1细胞凋亡。与空白对照组比较，加入H.pylori后，GES-1细胞凋亡率增加[（5.52±1.31）%比（28.96±3.14）%）]，差异有统计学意义（t＝－11.94，P〈0.05）。铝碳酸镁可阻止H.pylori对细胞的吸附，抑制H.pylori所致的细胞凋亡。与H.pylori损伤组比较，加用铝碳酸镁后GES-1细胞凋亡率下降[（28.96±3.14）%比（19.38±1.91）%]，差异有统计学意义（t＝4.52，P〈0.05）。 结论 铝碳酸镁对H.pylori有一定的体外抑制作用，并对多种菌体蛋白质的表达有调控作用。铝碳酸镁可减弱H.pylori对胃黏膜上皮细胞的吸附作用，保护细胞免受H.pylori损伤。

Objective To explore the mechanism underlying the inhibitory effects of Hydrotalcite on Helicobacter pylori （H.pylori）. Methods H. pylori international standard strain 26695 was resuscitated and incubated in Columbia solid culture medium and brucella broth. It was divided into blank control group, H. pylori injured group and H. pylori with Hydrotalcite group. H. pylori were treated by Hydrotalcite, and the viability changes of H. pylori were observed. The morphological changes of H. pylori were detected by Gram′s stain. The changes of mycoproteins expression of H. pylori were examined by Coomassie blue stain. The effects of H. pylori on cell apoptosis of gastric epithelial cell line GES-1 was compared between groups with and without Hydrotalcite. Subcellular structure and attachment ability of H. pylori on GES-1 cells were observed with transmission electron microscopy and scanning electron microscopy. T test was performed for statistical analysis. Results Hydrotalcite could inhibit the growth of H. pylori. However there were no significant morphological changes of H. pylori. Many mycoproteins were regulated by Hydrotalcite. Without Hydrotalcite, a large amount of H. pylori attached on the cell surface of GES-1 promoted the apoptosis of GES-1 cell. Compared with blank control group, the apoptosis rate of GES-1 cells increased after treated by H. pylori （（5.52±1.31）% vs （28.96±3.14）%）, and the difference was statistically significant （t=-11.94, P〈0.05）. Hydrotalcite could prevent the attachment of H. pylori on cells, which reduced the apoptosis caused by H. pylori （（28.96±3.14）% vs （19.38±1.91）%）, and the difference was statistically significant （t=4.52, P〈0.05）. Conclusion Hydrotalcite has inhibitory effects on H. pylori in vitro and play a role in regulation of many mycoproteins. Hydrotalcite can reduce the attachment of H. pylori on gastric epithelial cells, and protect cells from H. pylori injury.