食管间质成纤维细胞与食管癌细胞相互作用的体外研究

In vitro study on interaction between esophageal stromal fibroblasts and esophageal carcinoma cells

目的体外研究不同类型食管间质成纤维细胞与癌细胞株间接接触后其基因及生物学特性的改变。方法检测人食管正常间质成纤维细胞(NFs)、不典型增生间质成纤维细胞(AFs)及癌相关纤维母细胞(CAFs)与癌细胞株间接相互接触前后的波形蛋白(Vimentin)、α平滑肌肌动蛋白(α-SMA)、转化生长因子-β(TGF-β1)、人肝细胞生长因子(HGF)、基质金属蛋白酶(MMP)2、MMP9mRNA及食管癌细胞株的增殖细胞核抗原(PCNA)mRNA,并与食管癌细胞株进行侵袭实验。结果从NFs到AFs再到CAFs,Vimentin mRNA的表达无差别,α-SMA、TGF-β1、HGF、MMP2及MMP9mRNA的表达逐渐增强。在与食管癌细胞株间接接触后,NFs及AFs的α-SMA、TGF-β1、HGF、MMP2及MMP9 mRNA表达均上调,且差异有统计学意义(P<0.05),而CAFs的相应mRNA表达无明显变化。食管癌细胞株的PCNA mRNA在与NFs接触后,无变化;而与AFs和CAFs接触后,PCNA mRNA表达明显上调。结论食管癌细胞与食管间质成纤维细胞可影响对方的增殖活性及侵袭特性。

Objective To conduct the in vitro study on the changes of gene and biological characteristics of different esophageal interstitial fibroblasts after their indirect contact with esophageal cancer cell fines. Methods The mRNA levels of Vimentin,α- SMA,TGF-β1, HGF, MMP2 and MMP9 in normal esophageal interstitial fibroblasts（NFs）, atypical hyperplasia interstitial fibroblasts（AFs） and cancer related fibroblasts（CAFs） and PCNA mRNA in esophageal carcinoma cell line were detected after their indirectly mutual contact. The invasion test of carcinoma cell line was conducted. Results From NFs.AFs to CAFs,the expresmon of Vimentin mRNA had no difference. The mRNA expressions of β-SMA, TGF-β1, HGF, MMP2 and MMP9 were gradually increased. After indirectly mutual contact with esophageal carcmoma cell line. the mRNA expressions of α-SMA, TGF-β1, HGF, MMP2 and MMP9 in NFs and AFs were up-regulated,and the difference was statistically significant（P〈0.05）,while the related mRNA expression in CAFs had no obvious change. The expression of PCNA mRNA in esophageal carcinoma cell line had no change after contact with NFs, but the expression of PCNA mRNA was significantly up-regulated after interaction with AFs contacting with CAFs. Conclusion The esophageal carcinoma cells and esophageal interstitial fibroblasts could affect the proliferation activity and invasive characteristics of counterparts.