Smad2、Erk2及NF-kB在宫腔粘连组织中表达的临床研究

The expression of Smad2,Erk2,and NF-k B in endometrium of patient with intrauterine adhesion

目的检测宫腔粘连患者子宫内膜组织中与正常子宫内膜组织中Smad2、Erk2及NF-k B的表达情况,并分析其表达情况与宫腔粘连发生的相关性。方法采集2016年2月至2017年2月在哈尔滨市红十字中心医院妇产科就诊的58例宫腔粘连患者作为研究组,选择子宫内膜正常的患者30例作为对照组。应用逆转录-聚合酶链反应(RT-PCR)法检测Smad2、Erk2及NF-k B在以上组织中的表达。结果 Smad2和Erk2在研究组中的表达高于对照组(χ~2=17.683,P<0.01,χ~2=29.031,P=0.001),而NF-k B在研究组中的表达低于对照组,比较差异有统计学意义(χ~2=13.470,P=0.000)。研究组中Smad2和Erk2的表达与宫腔粘连的发生呈正相关。结论 (1)宫腔粘连组织中Smad2、Erk2及NF-k B的异常表达可能与宫腔粘连的发生有关。(2)在TGF-β/Smad信号通路中Smad2的过表达可能促进宫腔粘连的形成。

Objective To explore the Smad2, Erk2 and NF-kB expressions in endometrium of patient with intrauterine adhesion and normal people, and to analyze the correlation between their expressions and the occurrence of intrauterine adhesion. Methods 58 patients with intrauterineadhesion from February 2016 to February 2017 in the Harbin Red Cross Central Hospital were enrolled as the study group and 30 healthy women as the control group. RT-PCR was used to detect the expressions of Smad2, Erk2 and NF-kB in endometrium of the two groups. Results The expression of Smad2 and Erk2 in the study group （^2 = 17. 683, P =0. 000） was significantly higher than the control group（X2 = 29. 031, P = 0. 001）. But the expression of NF-kB was significantly lower than the control group （X2 = 13. 470, P = 0. 000）. The expressions of Smad2 and Erk2 had positive correlation in the study group. Conclusions The abnormal expressiona of Smad2, Erk2 and NF-kB in the endometrium may take part in the occurrence of intrauterine adhesion. The over-expression of Smad2 in TGF-p/Smad signaling pathway may promote the development of intrauterine adhesion.