响应面法优化刺梨总黄酮提取工艺

Optimization of Extraction Technology for Total Flavonoids from Rose roxburghii by Response Surface Methodology

为了优化刺梨总黄酮的提取工艺,在单因素试验的基础上,根据中心组合试验设计原理,设计三因素三水平的响应面法试验,确定刺梨总黄酮的最佳提取条件。优化得到的提取工艺为:以65%乙醇为提取溶剂,回流提取时间115.5 min,料液比为1∶71(g/m L),在此条件下总黄酮的提取率为10.76%。

bTo optimize the extraction technology for total flavonoids from Rose roxburghii. According to the re-sults of the single-factor experiment and the central composite experimental design principles,a response sur-face methodology which had three factors and three levels was designed to optimize the extraction technology fortotal flavonoids form Rose roxburghii. The effect of different drying methods（drying under the sun, drying in theshade, oven drying, microwave drying and freeze drying） on extraction rate was analyzed comparatively under theoptimal conditions. The extraction rate of total flavonoids at 10.76 % was achieved under the optimal conditions：65 % ethanol was used as extraction solvent with the ratio of1 ： 71 （g/mL）raw material to liquid, the reflux ex-traction time was 115.5 min.