喹烯酮与鸡鸭血浆蛋白结合率的检测

Determination of Plasma Protein Binding Rates of Quinocetone in Chicken and Duck

建立检测鸡鸭血浆中喹烯酮的方法,并测定其与鸡鸭血浆蛋白的结合率。采用平衡透析法测定喹烯酮的血浆蛋白结合率;血浆样品采用乙腈沉淀蛋白后用HPLC测定喹烯酮含量,同时采用HPLC测定透析外液中喹烯酮的含量,计算喹烯酮的血浆蛋白结合率,并对检测方法的方法学进行验证。建立的测定鸡鸭血浆中喹烯酮的方法,喹烯酮添加浓度为0.3、1和2μg/mL时,回收率范围为86.5%~92.2%,相对标准偏差均低于5%。喹烯酮在不同浓度的鸡鸭血浆中的平均蛋白结合率分别为40.53%±1.09%和46.88%±1.25%。喹烯酮药物浓度在0.5~8μg/mL的浓度范围内其血浆蛋白结合率无显著差异,其蛋白结合呈现非浓度依赖性。上述结果表明,喹烯酮在鸡鸭中应用时不易发生血浆蛋白结合介导的药物相互作用,药物的安全性较高。

Objective： To establish an assay method for the determination of quinocetone in poultry plasma, and to determine the plasma protein binding rate of quinocetone. Method： The equilibrium dialysis method was used to determine plasma protein binding rate of quinocetone invitro; the biological samples was extracted with acetonitrile, the analytes was separated by high performance liquid chromatography and the detection methodology is verified. Results： A method for the determination of quinocetone in the plasma was established and the linear range was 0.3-10 μg/mL, correlation coefficient r〉0. 999 5; average recovery rates around 86 5%-92 2% in spiked concentration of 0.3、1 and 2 μg/mL, and the relative standard deviation were below 5%. The average plasma protein binding rate of quinocetone in chicken and duck were 40 .53%±1.09% and 46 .88%±1. 25%. There was no significant difference between the plasma protein binding rate of quinocetone in the concentration range of 0. 5-8 μg/mL. The result shows that the probability of drug interaction is low and the application of quinocetone in poultry has high security.