芹菜素在Caco-2细胞的摄取研究

Uptake Study on Apigenin in Caco-2 Cells

目的研究芹菜素在Caco-2细胞的摄取特性。方法采用Caco-2单层细胞模型,研究芹菜素在不同时间、浓度、温度、pH值、P-糖蛋白抑制剂条件下对细胞摄取的影响,采用HPLC测定芹菜素在细胞中的含量。结果芹菜素在0.33~80μg/m L范围内线性关系良好(r=0.999 4)。芹菜素在Caco-2细胞的摄取呈现一定的时间、浓度和pH值依赖性,且P-糖蛋白抑制剂不能增加其摄取量。结论该方法操作简单、灵敏,P-糖蛋白未参与芹菜素的转运过程,芹菜素在Caco-2细胞的摄取主要是被动转运。

Objective To study the uptake property of apigenin in Caco-2 cells. Methods Caco-2 cell monolayer model was used to research the effects of apigenin on cell uptake in different times, concentrations, temperatures, pH values, and P-gp inhibitors. HPLC was used to determine the content of apigenin in cells. Results The linearity of apigenin concentration curve was found in good linear range of 0.33–80 μg/mL （r=0.999 4）. Uptake determination of apigenin in Caco-2 cells depended a certain of time, concentration and pH values, and P-gp inhibitors could not increase the apigenin uptake. Conclusion This method is simple and sensitive. P-gp is not involved in apigenin transport process, and uptake of apigenin in Caco-2 cells is mainly passive transported.