肝细胞癌组织miRNA-155水平变化及其对预后的影响

Hepatic miRNA-155 levels in prognosis of patients with hepatocellular carcinoma after hepatectomy

目的观察肝细胞癌(HCC)组织miRNA-155水平变化,并分析其对患者预后的意义。方法 2013年1月~2014年5月我院诊治的50例HCC患者,取手术切除的癌组织,另取50例正常肝组织作为对照组,采用荧光定量PCR法检测组织miRNA-155水平。结果正常肝组织miRNA-155水平(1.06±0.1)明显低于HCC癌组织,10例Ⅰ级、15例Ⅱ级、12例Ⅲ级和13例Ⅳ级HCC组织miRNA-155水平分别为(2.04±0.82)、(3.02±1.20)、(4.20±1.84)和(6.24±2.36);miRNA-155低水平HCC患者生存率(24.00%)明显高于miRNA-155高水平患者(9.24%,P<0.05);在不同分级HCC(14.26月对9.02月)、不同肿瘤大小(15.68月对10.02月)、肿瘤是否转移(8.20月对14.46月)、是否静脉侵犯(9.36月对14.36月)、肿瘤分化程度(15.20月对7.84月)和miRNA-155水平高低(7.86月对16.32月)患者间生存期均存在明显差异(P<0.05);肿瘤大小(>5 cm,HR=1.46,P=0.038)、肿瘤转移(是,HR=1.72,P=0.026)、静脉侵犯(是,HR=1.46,P<0.001)、肿瘤分化(高分化,HR=1.33,P=0.012)和miRNA-155(高水平,HR=1.65,P<0.001)均为影响HCC患者预后的独立危险因素(P<0.05)。结论肝癌组织miRNA-155水平明显高于正常肝组织,且高水平的miRNA-155为HCC患者预后的独立危险因素。结果表明,miRNA-155水平高低可评估HCC患者预后,可考虑将其作为评估HCC患者预后的独立预测因子之一。

Objective To investigate the levels of hepatic miRNA-155 in prognosis of patients with hepatocellular carcinoma （HCC）. Methods Cancerous tissues in 50 patients with HCC and 50 normal liver tissue samples in our hospital between January 2013 and May 2014 were obtained. The miRNA levels in cancerous and liver tissues were detected by RT-PCR,and the relationship between miRNA-155 and prognosis was conducted. Results miRNA-155 in normal liver tissues （1.06±0.1） were lower than in FICC,and the miRNA-155 levels increased as HCC grading increased （P〈O.05）;the survival rate （24.00%） in patients with low hepatic miRNA-155 level was significantly higher than that in with high miRNA-155 level （9.24%,P〈0.05）; the survival in patients was significantly different between different HCC classification （14.26% vs. 9.02%）,tumor size （15.68% vs. lO.02%）,tumor metastasis （8.20% vs. 14.46%）,venous invasion （9.36% vs. 14.36%）,tumor differentiation （15.20% vs. 7.84%） and different levels of miRNA-155（7.86% vs. 16.32%,P〈O.O5）;the tumor size （〉5 cm,HR =l.46,P=0.038）,tumor metastasis （HR =l.72,P=0.026）,venous invasion （HR =1.46,P〈0.001 ）,tumor classification （HR=l.33,P=0.012） and miRNA-155 levels （HR=l.65,P〈0.001） were the independent risk factors for poor prognosis （P〈0.05） in patients with HCC. Conclusion miRNA-155 levels in liver cancer tissues is obviously higher than in normal liver tissues,and the high miRNA-155 levels is independent risk factor in patients with HCC.