温阳益气方对便秘模型大鼠结肠水通道蛋白3、8表达及肠道动力的影响

Effect of Yang-warming and Qi-tonifying Recipe on Colonic Aquaporin 3,8 Expression and Intestinal Motility of Loperamide-induced Constipation Rats

【目的】观察温阳益气方对慢传输型便秘模型大鼠水通道蛋白(AQP)3、8的影响,探讨其治疗便秘的机制。【方法】采用洛哌丁胺灌胃法复制慢传输型便秘大鼠模型。将40只大鼠分为造模组(N=30),正常对照组(N=10);造模成功后再将造模大鼠随机分为模型组、温阳益气方组和莫沙必利组,每组10只,并给予相应的药物治疗,连续治疗2周。治疗结束后,采用碳末推进试验检测大鼠结肠传输功能,分别采用免疫组化法及实时荧光定量聚合酶链反应(q PCR)法检测AQP3和AQP8的表达。【结果】与正常对照组比较,便秘模型组碳末推进率明显降低,AQP3、AQP8蛋白和m RNA表达水平均显著升高(P<0.05或P<0.01)。与模型组比较,温阳益气方组及莫沙必利组炭末推进率显著升高,AQP3、AQP8的蛋白和m RNA表达水平均显著降低(P<0.05),但2组间比较,差异无统计学意义(P>0.05)。【结论】温阳益气方可能是通过降低肠道AQP3、AQP8的表达,减少肠腔液体重吸收,增加肠道内粪便含水量,起到治疗慢传输型便秘的作用。

Objective To observe the effect of Yang-warming and Qi-tonifying Recipe（YQP）on aquaporin 3（AQP3）and AQP8 in rats with slow transit constipation,and to explore its therapeutic mechanism. Methods Forty rats were randomly divided into modeling group（N=30）and normal control group（N = 10）. After successful modeling by gastric gavage of loperamide,the modeled rats were randomly divided into model group,YQP group and Mosapride group,10 rats in each group,and were separately treated with corresponding medicine for 2 weeks. After treatment,the colonic transit function was measured by carbon propelling test. The protein levels of AQP3 and AQP8 were detected by immunohistochemistry and their m RNA expression levels were detected with real-time fluorescence polymerase chain reaction（q PCR）. Results Compared with the normal control group,the propelling rate of carbon particle in the model group was decreased,and the protein and m RNA expression levels of AQP3 and AQP8 were significantly increased（P 0.05 or P 0.01）. Compared with the model group,the propelling rate of carbon particle of YQP group and mosapride group was significantly increased,and the protein and m RNA expression levels of AQP3 and AQP8 were significantly decreased（P 0.05）,but there was no significant difference between YQP group and mosapride group（P 0.05）. Conclusion YQP had therapeutic effects on loperamide-induced constipation through decreasing the expression of AQP3 and AQP8 in the intestine,reducing the reabsorption of intestinal fluid,and increasing the fecal water content.