结直肠癌细胞增殖核抗原及生长抑制因子4表达的关系及预后因素

Relationship between Ki-67 and ING4 expression and their prognostic significance in colorectal cancer

目的探讨细胞增殖核抗原(Ki-67)和生长抑制因子4(ING4)在结直肠癌发病机制中的作用及其对患者预后的影响。方法应用免疫组织化学法检测71例结直肠癌组织和30例癌旁正常组织中Ki-67、ING4的表达情况,分析其表达与临床病理特征及预后的关系。结果 Ki-67在癌组织中阳性表达率(85.9%)高于癌旁组织(6.7%)(P<0.05),ING4在癌组织中阳性表达率(56.3%)低于癌旁组织(90%)(P<0.05)。Ki-67表达与肿瘤浸润深度、有无淋巴结转移、淋巴结转移数目、TNM分期有关(P<0.05),ING4表达与分化程度、有无淋巴结转移、淋巴结转移数目、TNM有关(P<0.05)。Ki-67、ING4在结直肠癌组织中表达呈负相关性(r=-0.428,P<0.05)。Ki-67高表达组和低表达组术后5年生存率分别为63.9%、85.5%,差异有统计学意义(P<0.05);ING4阳性组和阴性组术后5年生存率分别为97.4%、45.2%,差异有统计学意义(P<0.05)。Cox多因素分析结果显示,TNM分期、ING4表达情况均为影响患者预后的独立因素。结论 Ki-67、ING4可能参与直肠癌的发生和发展,ING4是影响结直肠癌患者预后的独立危险因素。

Objective To investigate the expression of Ki-67 and ING4 and their influence on survival in patients with coloreetal cancer. Methods The expressions of Ki-67 and ING4 proteins were detected by immunohistochemistry in coloreetal cancer tissues from 71 cases of colorectal cancer and normal colorectal tissues from 30 corresponding para-cancerous normal tissues. The correlations between the expressions of Ki-67, ING4 and clinicopathological parameters and prognostic significance were also analyzed. Results The colorectal cancer tissues＇ s positive expression rates of Ki-67 was 85.9%, which was significantly higher in innormal tissue group （6. 7% ） （P 〈 0. 05 ）, The colorectal cancer tissues＇ s positive expression rates of ING4 was 56.3% , which was significantly lower in innormal tissue group（90% ）（P 〈0. 05 ）. Ki-67 expressions in eolorectal cancer tissues were correlated with infiltration depth,lymph node metastasis, number of lymph node metastasis and TNM staging （ P 〈 0. 05 ）. ING4 expressions in colorectal cancer tissues were correlated with histological grade, lymph node metastasis, number of lymph node metastasis and TNM staging（ P 〈 0.05 ）. Ki-67 was negatively correlated with ING4 （ r = - 0. 428 ,P 〈 0. 05 ）. The 5-year survival rate in the Ki-67 positive group （63.9%） was lower compared with the Ki-67 negative group （85.5%） （P 〈0. 05）. The 5-year survival rate in the ING4 positive group（97.4% ） was higher compared with the ING4 negative group （45.2%） （P 〈 0. 05 ）. The multivariate analysis showed that TMN stage and ING4 are independent prognostic factors in colorectal cancer. Conclusion Ki-67 and ING4 may be involved in the occurrence and development of colorectal cancer, and ING4 is independent factors affecting the prognosis in patient with colorectal cancer.