黄曲霉和米曲霉的多相鉴定方法

The polyphasic taxonomy for distinguishing Aspergillus flavus and A. oryzae

【背景】黄曲霉(Aspergillus flavus)和米曲霉(Aspergillus oryzae)形态特征相近,基因组高度相似,较难区分。【目的】旨在总结一套准确鉴别二者的分类方法。【方法】利用22株标准菌株对传统形态学、产毒培养基、酶联免疫毒素检测、系统发育分析、产毒基因检测等5种鉴别方法分别进行验证。【结果】各鉴定方法的结果存在异同,单一的鉴定方法容易出现假阴性或假阳性结果。【结论】利用单一方法区分黄曲霉和米曲霉具有潜在风险,多相鉴定方法可以准确鉴别二者。

[Background] The morphology and genome between Aspergillus flavus and A. oryzae were highly similar, which made it very difficult to distinguish with each other. [Objective] The aim of this study was to establish an accurate method for distinguishing A. flavus with A. oryzae. [Methods] Twenty-two standard strains were used to study the five traditional identification methods of A. flavus and A. oryzae, including the traditional morphological identification method, enzyme linked immunosorbent assay（ELISA）, the toxin-secreting culture medium method, the phylogenetic tree method and the detection method for toxin genes. [Results] There are differences or complements among the results of different methods. [Conclusion] A single method used to distinguish the two species would have a potential risk, while a polyphasic approach could resolve this problem.