转录因子E2F1在外阴鳞癌中的表达及意义

Expression and clinical significance of E2F1 in vulvar squamous cell carcinoma

目的:探讨转录因子E2F1在外阴鳞癌(VSCC)中的表达情况及其临床意义。方法:采用PCR(qRT-PCR)、Western blot和免疫组化检测30例VSCC及对应癌旁组织中E2F1的水平,分析E2F1表达与临床病理参数的关系。结果:VSCC组织中E2F1的mRNA相对表达量为(1.27±0.04),高于癌旁外阴组织的E2F1水平(0.84±0.06)(P<0.05);VSCC组织中E2 F1的蛋白相对表达量为(1.16±0.04),高于癌旁外阴组织的E2F1水平(0.93±0.03)(P<0.05);免疫组化示VSCC组织中E2F1的阳性表达率(56.67%,17/30)明显高于癌旁外阴组织(33.33%,10/30)(P<0.05),且其阳性表达率与FIGO分期有关(P<0.05)。结论:E2F1在VSCC组织中表达上调,且与FIGO分期有关,提示E2F1可能与VSCC的发生、发展有关。

Objective：To investigate the expression level of E2F1 and its physiological significance in vulvar squamous cell carcinoma（VSCC）.Methods：Semi-quantitative RT-PCR,Western blot and immunohistochemistry were used to analyze E2F1 mRNA and protein expressions in 30 cases of VSCC and adjacent normal tissues.Clinical records and the pathology of the frozen specimens were collected to analyze the relationship between E2F1 and clinical pathology.Results：Semi-quantitative RT-PCR showed the average E2F1 mRNA expression levels were （1.27±0.04） and （0.84±0.06） in VSCC tissues and adjacent normal tissues（P〈0.05）.Western blot showed average E2F1 protein expression levels were （1.16±0.04） and （0.93±0.03） in VSCC tissues and adjacent normal tissues（P〈0.05）.Immunohistochemistry showed the protein positive expression rate of E2F1 in VSCC tissues （56.67%,17/30） was significantly higher in VSCC than that in normal tissues （33.33%,10/30） （P〈0.05）.The mRNA positive expression rate of E2F1 was positively correlated with FIGO stage.Conclusion：The up-regulation of E2F1 in VSCC was found to be associated with FIGO stage,E2F1 might be associated with the tumourigenesis and development of VSCC.