红河州HIV/HCV合并感染者HCV基因亚型多样性及种系进化研究

Genotypes diversity and phylogenetic analysis of HCV in HIV/HCV co-infection patients in Honghe

目的探讨云南省红河州地区HIV/HCV合并感染者HCV基因亚型多样性及种系进化情况,为本地区HIV/HCV合并感染者合理的抗病毒治疗提供科学依据,同时监测该类患者体内的HCV是否存在遗传进化的新变异。方法扩增55名红河州内HIV/HCV合并感染的HCV病毒载量阳性的丙型肝炎患者C/E1和NS5B 2个基因位点,通过测序分析和种系进化分析以明确研究对象所感染的HCV病毒的基因型和亚型。结果综合2个外显子序列分析,结果显示红河州HIV/HCV合并感染患者的HCV以3b亚型最多见(40.0%),其次是3a(20.0%)、6a(16.4%)、6n(12.7%)、1b(9.1%)、双重感染(1.8%)。课题组做本地区HCV基因亚型遗传进化分析时发现红河州地区流行的6a亚型毒株序列均与越南的HCV 6a亚型毒株的参考序列丛集。结论 HCV 6a亚型是越南和红河州内静脉吸毒人群(intravenous drug uses,IDUs)中高流行的病毒亚型,由于与越南接壤,红河州可能是HCV 6a亚型通过静脉吸毒途径由越南传入中国流行的一个传入点,进而再传入昆明或中国的其它地区。

Objective To investigate the diversity of HCV genotype and phylogenesis in HIV/HCV co-infection patients in Honghe of Yunnan province. On one hand, it will provide reasonable therapy for the patients; on the other hand, we can supervise new variants of HCV in this area. Methods 55 HIV/HCV co- infection patients, who tested sere-positive and HCV-RNA positive, were recruited into this study. 2 regions of HCV gene, C/E1 and NS5B were PCR- amplified, sequenced and phylogenetically analyzed. Results Sequencing results of the 2 coding regions showed that subtype 3b was the most predominant （40.0%）, followed by 3a （20.0%）, 6a （16.4%）, 6n （12.7%）, lb （9.1%）, and lastly dual-infection （1.8%）. Phylogentic analysis showed that subtype 6a strain from Honghe was clustered with reference sequence from Vietnam. Conclusion HCV 6a is a high prevalent subtype in intravenous drug users （IDUs） both in Vietnam and Honghe. Due to bordering Vietnam, Honghe might be one of the dissemination entrance points of HCV subtype 6a from Vietnam to China with the main transmission route of IDU.