辣蓼挥发油GC-MS分析及抗菌效果考察

GC-MS analysis and antibacterial effect of volatile oil in Polygonum hydropiper

目的分析和鉴定辣蓼中挥发油的化学成分,并检测其抗菌效果,以便更合理地开发利用辣蓼资源。方法采用水蒸汽蒸馏法提取辣蓼挥发油,气相色谱-质谱联用法分离和鉴定其化学成分,并用峰面积归一化法测定了各组分的相对含量。通过体外抗菌实验检测辣蓼挥发油的抗菌效果。结果从辣蓼挥发油中分离并鉴定出39个组分,占挥发油总量的82.31%。体外抗菌实验表明,其对多种致病菌起到良好的抗菌效果。结论本实验为进一步研究辣蓼挥发油奠定理论基础,为深度开发和利用辣蓼资源,提供科学依据。

Objective： To analyze and identify the chemical constituents of volatile oil in Polygonum hydroplper, and to test its antibacterial effect,so as to develop and utilize the resources of P.hydropiper more reasonably. Method： The volatile oil of P.hydropiper was extracted by steam distillation. The chemical constituents were isolated and identified by GC-MS,and the relative content of each component was determined by peak area normalization method.The antibacterial effect of volatile oil of P.hydropiper was detected by antibacterial test in vitro. Result：39 components were separated and identified from the volatile oil of P.hydropiper,which accounted for 82.31% of the total volatile oil.Antibacterial experiments in vitro showed that it has good antibacterial effect on various pathogenic bacteria.Conclusion：This experiment lays a theoretical foundation for further research on the essential oil of P.hy- dropiper, and provides a scientific basis for further exploitation and utilization of P.hydropiper.