基于化学衍生化的靶向脂质组学质谱分析研究进展

Advances in chemical derivatization-based targeted lipidomics by mass spectrometric analysis

脂质组学研究与一些重大疾病(如心血管病、糖尿病、肿瘤等)密切相关,是当今生命科学研究的重要领域.质谱分析技术是目前脂质组学研究的核心手段之一.化学衍生化策略可显著提高基于鸟枪法或液相色谱-质谱联用方法的脂质组学分析性能,近年来已有一些相关研究报道.本文重点介绍了基于化学衍生化的质谱分析方法在靶向脂质组学研究中的进展,并提出了问题和展望.希望在衍生化试剂的设计与合成,特别是具有显著优势的稳定同位素标记衍生试剂开发,以及新的衍生化策略和相应的质谱分析方法的开发与应用方面起到一定的推动作用.

Lipidomics plays a significant role in the related studies of some serious diseases such as cardiovascular disease, diabetes and tumor, that has become a hot area of life science. Mass spectrometry is currently one of the core methods for lipidomics study. The strategy of chemical derivatization can remarkably improve the performance of mass spectrometric technologies for both shotgun and liquid chromatography-mass spectrometry-based lipidomics, and some interesting results have been reported. Therefore, this review will focus on the research progress in chemical derivatization-based targeted lipidomics by shotgun method and liquid chromatography-mass spectrometry, and the current problems as well as prospects in this area are also discussed. It should be helpful for the design and synthesis of new derivatization reagents, especially the stable isotope labeling derivatization reagents. The development and applications of new chemical derivatization strategies will provide useful techniques for lipidomics research.