胃泌素及线粒体途径凋亡相关蛋白在胃癌组织中的表达及意义

Expressions of gastrin and apoptosis-associated proteins involved in mitochondrial pathway in gastric cancer tissues and the clinical significance

目的研究胃泌素及线粒体途径凋亡相关蛋白在胃癌组织中的表达,探讨其相关性及临床意义。方法通过组织芯片技术和免疫组织化学染色法,检测胃癌组织及癌旁组织内胃泌素及线粒体途径凋亡相关蛋白Bcl2、胱天蛋白酶9(caspase-9)及caspase-3的表达,采用Spearman等级相关分析及卡方检验分析它们之间的相关性及其与临床病理特征的关系。结果胃泌素及Bcl2在胃癌组织中的阳性表达率均明显高于癌旁组织;caspase-9及caspase-3在胃癌组织中的阳性表达率均显著低于癌旁组织。其中,胃泌素与Bcl2在胃癌组织中的表达呈显著正相关(r=0.237)。胃泌素的表达与肿瘤部位相关,Bcl2的表达与肿瘤大小、TNM分期及淋巴结转移有关。结论胃癌组织胃泌素与Bcl2高表达且与临床病理特征有一定相关性。

Objective To investigate the expressions and correlations of gastrin and apoptosis-associated proteins involved in mitochondrial apoptotic pathway in gastric cancer tissues,and explore their clinicopathological characteristics.Methods The tissue chip technology and immunohistochemistry（ IHC） were used to detect the expressions of gastrin and apoptosis-associated proteins（ Bcl2,caspase-9 and caspase-3） in human gastric cancer tissues and their paracancerous tissues. The correlations of these markers and their clinicopathological characteristics were analyzed using Spearman rank correlation analysis and Chi-square test. Results The expressions of gastrin and Bcl2 in gastric cancer tissues were significantly higher than those in the corresponding paracancerous tissues, whereas the expressions of caspase-9 and caspase-3 in gastric cancer tissues was significantly lower than those in the corresponding tissues. There was a significant positive correlation between the expressions of gastrin and Bcl2 in gastric cancer tissues（ r = 0. 237）. The expression of gastrin was associated with tumor position,and the expression of Bcl2 was associated with tumor size,TNM stage and lymph node metastasis. Conclusion Gastrin and Bcl2 are highly expressed in gastric cancer tissues,and they are correlated with the clinicopathologic features.