新型鸭呼肠孤病毒病浓缩灭活疫苗的制备及免疫原性分析

Efficacy Evaluation of a Concentrated Inactivated Vaccine against Novel Duckling Reovirus Infection

为研制高效的新型鸭呼肠孤病毒病(NDRV)灭活疫苗,分别采用病毒尿囊液和浓缩后病毒尿囊液为抗原,经甲醛灭活处理后与常规油佐剂制成灭活疫苗,并进行种鸭免疫后血清学检测、雏鸭免疫保护及安全试验。结果表明,浓缩疫苗安全性好,能明显提高疫苗的免疫效果,高峰期抗体效价的免疫持续期比常规疫苗长;浓缩疫苗免疫雏鸭后的免疫保护性达100%。以上研究表明,NDRV浓缩疫苗安全、高效,在雏鸭上具有良好的免疫保护效果,具有良好的市场前景。

In this paper, an experimental concentrated inactivated vaccine and a conventional reactivated vaccine against Novel Duckling Reovirus （NDRV） infection were prepared. After one single-dose immunization in adult Cherry Valley ducks, the serum samples collected in a period of 13 weeks were tested for antibodies by serum neutralisation （SN） method. The protective effects against NDRV infection were observed in 3-day-old Cherry Valley ducklings and mule duckling after a single-dose immunization. The serological results showed that concentrated inactivated vaccine was much more immunogenic than the conventional inactivated vaccine, with a much higher SN antibody titers at all test time points. The concentrated inactivated vaccine was highly protective against NDRV challenge at 7-day post-vaccination with a 100% protective efficacy, as compared to 40%- 60% protective efficacy for conventional inactivated vaccine. Taken together, this experimental concentrated inactivated vaccine exhibited immune efficiency much superior to conventional inactivated vaccine and might be a useful vaccine against NDRV infection.