凋亡调节蛋白Bcl-2和Apaf-1在乳腺癌中的表达及临床意义

Expression and clinical significance of apoptosis regulatory proteins Bcl-2 and Apaf-1 in breast cancer

目的:研究Bcl-2和Apaf-1蛋白在乳腺癌组织及正常乳腺组织中的表达,并探讨二者与相关临床病理因素的关系及二者表达的相关性。方法:选取2014年1月至2015年12月患者相关材料完整的乳腺癌存档蜡块60例,应用免疫组织化学法分析检测Bcl-2和Apaf-1的表达情况同时分析两者表达的相关性。结果:Bcl-2蛋白的阳性表达率在乳腺癌组织内为41.67%(25/60),在癌旁组织内为13.33%(4/30),前者高于后者(P<0.05);而Apaf-1在乳腺癌组织中阳性表达率为21.67%(13/60),低于癌旁组织中的阳性表达73.33%(22/30)(P<0.05);Bcl-2蛋白、Apaf-1的表达与BMI指数及年龄不存在明显相关性(P>0.05),但与肿瘤大小、临床分期与淋巴结是否转移存在相关性(P<0.05),二者在组织中表达呈负相关(r=-0.499,P=0.001)。结论:Bcl-2和Apaf-1在乳腺癌的发展和侵袭中发挥着重要作用,且有可能作为临床判断乳腺癌预后的标志物。

Objective：To investigate the expression of Bcl -2 and Apaf-1 protein in breast cancer tissues and normal breast tissues, and to explore the relation-ship between the expression and correlation of the two proteins and clinicopathological factors. Methods ：60 cases of breast cancer paraffin blocks with the complete related materials from January 2014 to December 2015 were selected. Immunohistochemical method was used to detect the expression of Bcl - 2 and Apaf - 1 and analyze their correlation. Results：The positive expression rate of Bc1-2 protein in breast cancer tissues was 41.67 % （25/60）, and in the adjacent tissues was 13.33 % （4/30） ; the former was significantly higher than the latter （ P 〈 0.05 ）. The positive rate of Apaf - 1 in breast cancer was 21.67 % （ 13/60）, which was significantly lower than that in the adjacent tissues （73.33 %, 22/30） （ P 〈 0.05 ）. There was no significant correlation between the expression of Bcl - 2 and Apaf - 1 protein and the BMI index and age （ P 〉 0.05 ）. However, there was a significant correlation in tumor size, clinical stage and lymph node metastasis （ P 〈 0.05 ） and there was a negative correlation between the expression of the two proteins （ r = - 0.499, P 〈 0.05 ）. Conclusion： Bcl - 2 and Apaf - 1 play an important role in the development and invasion of breast cancer, and may be used as a marker to judge the prognosis of breast cancer.