苜蓿14-3-3基因家族的鉴定与进化和特征分析

Identification, Evolution and Characteristic Analysis of 14-3-3 Gene Family in Medicago

蛋白质磷酸化是所有真核生物中都存在的重要信号传导机制,在生命过程的多个基础性环节,包括细胞分裂、分化、凋亡等,都起着中心的调控作用。14-3-3蛋白是在所有真核生物中都存在的,识别和调控磷酸化蛋白质活性,在磷酸化信号传导网络中是一个基础性的中心蛋白。我们筛选鉴定了蒺藜苜蓿全基因组中和天蓝苜蓿叶片转录组中的14-3-3基因,并对这些基因的结构、染色体定位、进化和在蒺藜苜蓿各个器官及不同胁迫处理下的表达情况进行分析,推测各个基因可能的功能。从蒺藜苜蓿的基因组,鉴定出11个14-3-3基因,这些基因在大豆中均存在直系同源基因。其中10个基因有基因芯片的表达证据。从天蓝苜蓿的叶片转录组中,筛选鉴定出6个14-3-3基因。蒺藜苜蓿14-3-3基因在各个组织和器官中特异性的表达,并可响应外界生物和非生物胁迫。本研究为进一步阐明蒺藜苜蓿中14-3-3基因铺平了道路,指明了方向。

Protein phosphorylation is an important signal transduction mechanism in all eukaryotes, and plays a central regulatory role in many fundamental processes of cell life, including cell division, differentiation, and apoptosis. 14-3-3 protein is present in all eukaryotes, recognizes and regulates phosphorylated protein activity,which is a central protein in the phosphorylated signaling network. The 14-3-3 genes in the genome of Medicago truncatula and the transcriptome of Medicago sativa were screened and identifiedin this research, and the structure,chromosome location, evolution and expression of these genes in various organs and different stress treatments of M. truncatula were studied. Moreover, the possible function of each gene was analyzed.Eleven 14-3-3 genes were identified from the genome of Medicago truncatula, and these genes were found to be orthologous to soybean14-3-3 genes, of which 10 genes had gene expression evidence. Six 14-3-3 genes were identified from leaf transcriptions of alfalfa. The gene of 14-3-3 of Medicago truncatulawas specifically expressed in various tissues and organs, and could respond to external cbiotic and abiotic stresses. This study might pave the way for the further elucidation of 14-3-3 gene in Medicago truncatula.