摘要
目的探讨丁苯酞对慢性低灌注大鼠血清及脑组织中B淋巴细胞瘤/白血病-2(Bcl-2)、Bcl-2相关蛋白X(Bax)表达的影响。方法选择Wistar大鼠45只,随机分为正常对照组、模型组、丁苯酞组,每组各15只。模型组与丁苯酞组以结扎动脉远近端的方法制备大鼠慢性低灌注模型,丁苯酞组于造模后采用丁苯酞注射液进行注射,正常对照组和模型组给予注射等量的生理盐水,于3 d后采用HE染色观察病理形态学改变,酶联免疫吸附法检测各组大鼠血清及脑组织匀浆液中Bcl-2、Bax的表达情况。结果慢性低灌注后3 d,与正常对照组比较,模型组血清及脑组织匀浆液Bcl-2表达下降、Bax表达上升(P<0.05);与模型组比较,丁苯酞组血清及脑组织匀浆液Bcl-2表达上升,Bax表达下降(P<0.05)。结论 Bcl-2低表达,Bax高表达可能在慢性低灌注病理损伤过程中起了一定的作用。丁苯酞处理可以增加Bcl-2的表达、降低Bax的表达,这可能是丁苯酞修复慢性低灌注损伤的分子机制之一。
Objective To investigate the effect of butylphthalide on the expression of B lymphocyte tumor/leukemia-2（ Bcl-2） and Bcl-2 related protein X（ Bax） in serum and brain tissue of chronic hypoperfusion rats. Methods A total of 45 Wistar rats were randomly divided into the normal control group,model group and butylphthalide group,with 15 rats in each group. The rats in the model group and butylphthalide group were made into chronic low perfusion models using ligation method of proximal and distal artery,rats in the butylphthalide group were given butylphthalide injection after modeling,the others in the normal control group and the model group were given the same volume of saline injection. After 3 days,using HE staining to observe the pathological changes,and the expression of Bcl-2 and Bax in serum and brain tissue homogenates of rats were detected by ELISA. Results After 3 days of chronic low perfusion,compared with the normal control group,the expression of Bcl-2 reduced and Bax increased in the model group（ P〈0. 05）; compared with the model group,the expression of Bcl-2 increased and Bax reduced in the butylphthalide group（ P〈0. 05）.Conclusion Lowexpression of Bcl-2 and high expression of Bax may play a role in the pathological process of chronic hypoperfusion.Butylphthalide treatment can increase the expression of Bcl-2 and reduce the expression of Bax,which may be one of the molecular mechanisms of chronic hypoperfusion injury repaired by butylphthalide.
出处
《临床军医杂志》
CAS
2017年第12期1247-1249,共3页
Clinical Journal of Medical Officers
基金
陕西省自然科学基础研究计划项目(2014JM4131)
