Ezrin与磷酸化Ezrin蛋白在肾透明细胞癌中表达及意义

Expression of Ezrin and p-Ezrin( Thr-567) in human renal clear cell carcinoma and its significances

目的探讨Ezrin与磷酸化Ezrin(Thr-567)[p-Ezrin(Thr-567)]蛋白在肾透明细胞癌(RCCC)与癌旁正常肾组织的表达及其意义。方法收集120例RCCC与30例癌旁正常肾组织标本,采用免疫组化法检测Ezrin与p-Ezrin(Thr-567)蛋白的表达情况。结果 Ezrin蛋白在RCCC组织中的阳性与强阳性表达率分别为81.6%(98/120)与65.8%(79/120),在癌旁正常肾组织中分别为63.3%(19/30)与43.3%(13/30);p-Ezrin(Thr-567)蛋白在RCCC组织中的阳性与强阳性表达率分别为82.5%(99/120)与75.8%(91/120),在癌旁正常肾组织中分别为66.6%(20/30)与46.7%(14/30)。Ezrin与p-Ezrin(Thr-567)蛋白在RCCC组织中的表达明显高于癌旁正常肾组织,差异有统计学意义(P<0.05)。在RCCC组织中,伴有淋巴结转移的Ezrin与p-Ezrin(Thr-567)蛋白强阳性表达率分别为76.9%(20/26)与92.3%(24/26),无淋巴结转移的分别为63.8%(59/94)与63.9%(60/94),差异有统计学意义(P<0.05);伴有瘤栓形成的Ezrin与p-Ezrin(Thr-567)蛋白强阳性表达率分别为76.5%(13/17)与88.2%(15/17),无瘤栓形成的分别为61.2%(63/103)与59.2%(61/103),差异有统计学意义(P<0.05)。结论 Ezrin与p-Ezrin(Thr-567)蛋白在RCCC组织中高表达,尤其p-Ezrin(Thr-567)蛋白强阳性表达在伴有淋巴结转移或有瘤栓形成的RCCC组织中更加显著。p-Ezrin(Thr-567)蛋白可成为RCCC转移与侵袭的预测靶点,为临床治疗提供依据。

Objective To investigate the expression and significance of Ezrin protein and phosphorylated ezrin（ p-Ezrin）（ Thr-567） in human renal clear cell carcinoma（ RCCC） and adjacent normal renal tissues. Methods Immunohistochemistry was used to detect the expression of Ezrin protein and p-Ezrin（ Thr-567） protein in 120 cases of human RCCC and in 30 cases of adjacent normal renal tissues. Results The positive rate and strong positive rate of Ezrin protein expression in RCCC were 81. 6%（ 98/120） and 65. 8%（ 79/120）,respectively,in adjacent normal renal tissues were 63. 3%（ 19/30） and 43. 3%（ 13/30）,respectively; the positive rate and strong positive rate of p-Ezrin（ Thr-567） protein expression in RCCC were 82. 5%（ 99/120） and 75. 8%（ 91/120）,respectively,in adjacent normal renal tissues were 66. 6%（ 20/30） and 46. 7%（ 14/30）,respectively. The expression of Ezrin and p-Ezrin（ Thr-567）in RCCC tissues were significantly higher than that in adjacent normal renal tissues（ P〈0. 05）. In RCCC tissues with and without lymph node metastasis,the strong positive rate of Ezrin and p-Ezrin（ Thr-567） were 76. 9%（ 20/26） versus 63. 8%（ 59/94） and92. 3%（ 24/26） versus 63. 9%（ 60/94）,（ P〈0. 05）; in RCCC tissues with and without tumor embolism,the strong positive rate of Ezrin and p-Ezrin（ Thr-567） were 76. 5%（ 13/17） versus 61. 2%（ 63/103） and 88. 2%（ 15/17） versus 59. 2%（ 61/103）,（ P〈0. 05）. Conclusion Ezrin and p-Ezrin（ Thr-567） protein are highly expressed in RCCC tissues and the positive expression of p-Ezrin（ Thr-567） is more pronounced in RCCC tissues with lymph node metastasis or tumor embolism. The p-Ezrin（ Thr-567） protein can be a predictive target for RCCC metastasis and invasion,which provides a basis for clinical treatment.