舰载保存对去白细胞悬浮红细胞相关参数影响的研究

Experimental research on parameters of red blood cells in additive solution leukocytes reduced preserved in navy ship force

目的研究舰载保存去白细胞悬浮红细胞中红细胞计数、血红蛋白、红细胞压积、平均红细胞体积、红细胞分布宽度变异系数(RDW-CV)和红细胞分布宽度标准差(RDW-SD)参数的变化。方法根据《献血者健康检查要求》(GB 18467-2011),于出海前1 d,随机采集和制备去白细胞悬浮红细胞10人份(2 U)。每份血液分别取样,检测相关参数,作为航行前数据。然后每份血液等分为二,标记为对照组和试验组,分别储存于实验室和医疗船储血冰箱,于航行第21天取样进行去白细胞悬浮红细胞相关参数的检测。结果 (1)对照组和试验组的红细胞数量和血红蛋白浓度均低于航行前,红细胞数量两组间与航行前比较差异无统计学意义(P=0.319),而血红蛋白浓度变化较显著(P=0.002);(2)两组的红细胞压积和平均红细胞体积均高于航行前,红细胞压积在两组间与航行前比较变化大(P=0.015),而平均红细胞体积差异不明显(P=0.051);(3)两组的RDW-SD和RDW-CV均大于航行前,RDW-SD在两组间与航行前比较变化大(P<0.001),而RDW-CV差异不明显(P=0.528)。结论去白细胞悬浮红细胞在海上保存过程中,红细胞数量和血红蛋白浓度随保存时间延长而降低,红细胞压积、平均红细胞体积、RDW-SD和RDW-CV随保存时间延长而增大。远航对去白细胞悬浮红细胞相关参数有一定影响,但与常规储血冰箱(4±2)℃保存的变化趋势仍较一致。

Objective To study the changes in red blood cells（RBCs）,hemoglobin（Hb）,hematocrit（HCT）,mean corpuscular volume（MCV）,RBC distribution width variation coefficient（RDW-CV） and RBC distribution width standard deviation（RDW-SD） of leukocyte-poor red blood cells preserved in navy ship force on a long voyage.Methods According to the Requirement of Health Examination for Blood Donors（GB 18467-2011）,red blood cells in additive solution leukocytes reduced were randomly collected from ten healthy voluntary blood donors one day before sailing.Each blood sample was preserved.The relevant parameters were detected as data before sailing.Each blood sample was divided into two parts,one in test group and the other in control group.Samples were taken from the two groups of leukocyte-poor red blood cells for the sampling detection after 21 days of sailing respectively.Results（1）After sailing,the concentrations of RBCs and Hb declined in both groups,and the difference of RBC concentrations was not significant（P = 0.319）,but that of the concentrations of Hb was significant（P = 0.002）.（2）After sailing,the concentrations of HCT and MCV increased,but the change of HCT was significant（P = 0.015）,while that of MCV was insignificant（P = 0.051）.（3）After sailing,the concentrations of RDW-SD and RDW-CV were higher,but the change of RDW-SD was significant（P〈0.001）,while that of RDW-CV was not（P = 0.528）.Conclusion When leukocyte-poor red blood cells are preserved in navy ship force,the concentrations of RBC and Hb decrease,while the concentrations of HCT,MCV,RDW-SD and RDW-CV increase with the prolongation of preservation.A long voyage has some impact on the parameters of red blood cells in additive solution leukocytes reduced,as is the case with the conventional blood storage refrigerator（4 ± 2） ℃.