不同量级运输振动对悬浮红细胞质量影响的研究

Effect of simulated transportation vibration on suspended erythrocyte quality

目的研究运输过程不同量级振动对悬浮红细胞质量的影响,探索适宜的实验振动条件。方法使用电磁振动实验系统模拟不同运输振动,分别选择高速公路卡车振动环境(A)、履带车固定货物的窄带振动环境(B)及组合轮式车振动环境(C),水平、纵向、垂直三方向振动,累计振动1 h。选取储存11和26 d的悬浮红细胞各20袋,将相同储存时间的悬浮红细胞随机分为4组:A组、B组、C组、对照组。分别在振动前、后留取血样,检测游离血红蛋白(free hemoglobin,FHb)、乳酸脱氢酶(lactic dehydrogenase,LDH)、钾离子(K+),分析比较不同量级振动对悬浮红细胞质量的影响,以及不同储存时间悬浮红细胞经相同量级振动后的变化。结果悬浮红细胞振动后FHb、LDH变化值随振动量级的增强而逐渐升高,C组较其他量级振动变化显著(P<0.05);在3个振动量级间,K+变化值差异无统计学意义(P>0.05);同一量级振动后储存26 d的悬浮红细胞FHb、LDH变化值高于储存11 d的悬浮红细胞(P<0.05),但A组振动后FHb变化值及相同量级振动后K+变化值在不同储存时间红细胞间差异无统计学意义(P>0.05)。结论组合轮式车振动导致的悬浮红细胞振动损伤较高速公路卡车振动、履带车固定货物的窄带振动显著,组合轮式车振动环境更适于模拟极端陆地运输振动对悬浮红细胞振动损伤的研究;储存时间较长的悬浮红细胞经运输振动后FHb、LDH变化显著,储存时间可能是影响悬浮红细胞振动损伤的一个重要因素。

Objective To investigate the effect of vibration on the quality of suspended erythrocytes during transportation,and explore suitable experimental vibration conditions.Methods Three intensities（highway truck vibration,random vibration of fixed goods in a caterpillar,and combined wheeled vehicle vibration） were selected to simulate suspended erythrocyte transportation using electromagnetic vibration test system.Suspended erythrocytes of the same storage were randomly divided into three groups： highway truck vibration（group1）,random vibration of fixed goods in a caterpillar（group 2）,and combined wheeled vehicle vibration（group 3）.The suspended erythrocytes were stored for11 days and 26 days.The control group was stored with conventional methods.Suspended erythrocytes were vibrated for 1 hour,samples were collected before and after vibration,while free hemoglobin（FHb）,K＋,and LDH were tested.Results The changes in FHb and LDH after vibration were gradually increased with the magnitude of vibration（P〈0.05）.There was no significant difference in K＋between the three vibration levels.The increase in FHb in suspended erythrocytes stored for 26 days was higher than 11 days after random vibration of fixed goods in a caterpillar and combined wheeled vehicle vibration（P〈0.05）.There was no significant difference in the change in FHb between day（d） 26 and d 11 after highway truck vibration.Under the same magnitude of vibration,the change in LDH in the d 26 suspended erythrocytes was more significant than that of the d 11,and no significant difference was found in the change in K＋between d 26 and d 11.Conclusion The damage to suspended erythrocytes after combined wheeled vehicle vibration is more obvious than that by the other two vibrations.The vibration environment of combined wheel vehicles is more suitable for simulating the vibration damage to suspended erythrocyte vibration.Suspended erythrocytes with a longer storage time have significant changes in FHb and LDH after transportation vibration,and the length of storage time of suspended red blood cells might affect the vibration injury.