振动对不同储存时间悬浮红细胞影响的研究

Effects of vibration on red blood cells stored for different lengths of time

目的探讨悬浮红细胞运输过程中振动对不同储存时间悬浮红细胞游离血红蛋白(free hemoglobin,FHb)浓度、乳酸脱氢酶(lactic dehydrogenase,LDH)、钾离子(K^+)浓度的影响。方法分别选取采集后保存3、7、11、26、35 d的悬浮红细胞(2 U)各7袋,共计35袋,使用电磁振动实验系统模拟运输过程中产生的振动,选择振动参数:组合轮式车振动环境,随机振动,振动方向(水平、纵向、垂直三方向),每个方向振动1 h。红细胞分别在振动前、累计振动3 h后留取血样,检测FHb、LDH、K^+浓度变化。结果储存3、7、11、26、35 d的悬浮红细胞,振动后FHb较振动前显著升高(P<0.05);随储存时间的延长,FHb浓度表现为增加的趋势。储存3、7、11、26、35 d的悬浮红细胞,LDH含量随储存时间的延长而明显增加,振动3 h后与振动前比较,LDH显著升高(P<0.05);K^+含量变化有同样趋势(P<0.05)。结论运输振动及储存时间均对悬浮红细胞FHb、LDH、K^+含量的改变具有显著影响。

Objective To study the effect of vibration on free hemoglobin（FHb）,lactic dehydrogenase（LDH）,and K~＋concentration of red blood cells（RBCs） stored for different lengths of time.Methods Thirty-five bags of RBCs stored for different lengths of time（3,7,11,26,and 35 d,7 bags per group） were chosen as the target of research.A wheeled vehicle that could vibrate in three directions（horizontal,longitudinal,and vertical） was used to simulate RBC transportation in addition to an electromagnetic vibration test system.The RBC samples were prepared and vibrated for 0 h,or 3 h for detection and analysis of their FHb,LDH,and K~＋concentration.Results The FHb concentration was significantly increased after vibration（P〈0.05）,and tended to increase with the extension of storage time.LDH（P〈0.05） and K~＋concentrations（P〈0.05） were obviously increased with the length of storage（3,7,11,26,and 35 d）,and significantly increased after vibration of 3 h.Conclusion FHb concentration is increased after vibration,and the storage time could change FHb.The vibration and storage time have great effect on LDH and K~＋concentrations.