麻风树叶提取物体外抗蠕形螨活性及皮肤安全性的实验研究

Anti-demodecidosis activity and skin safety of Jatropha curcas.l leaves extract in vitro

目的探讨麻风树叶治疗蠕形螨病的应用价值。方法用80%乙醇热回流提取法提取麻风树叶提取液。取蠕形螨感染者面部皮脂,分离并鉴定蠕形螨备用。设不同浓度麻风树叶实验组、2%浓度的甲硝唑对照组和生理盐水对照组,进行体外抗螨实验。pH仪测定不同浓度麻风树叶提取物pH值。设麻风树叶实验组和75%乙醇对照组,用健康家兔进行皮肤刺激实验和急性皮肤毒性实验。结果 50、25、12 mg/ml麻风树叶组与2%甲硝唑组毛囊蠕形螨死亡时间分别为(1.55±0.67)min、(1.61±0.67)min、(2.47±0.80)min和(1.20±0.48)min。50、25 mg/ml麻风树叶组以及2%甲硝唑组两两之间差异无统计学意义(P均>0.05)。50、25、12 mg/ml麻风树叶提取液pH值分别为6.07±0.73、6.27±0.82、6.35±0.83,对家兔完整皮肤及破损皮肤刺激评分均为0,且无明显毒性。结论麻风树叶提取物具有较强的体外抗蠕形螨活性且具有皮肤安全性。

Objective To test the application value of the extract of Jatropha curcas.l leaves in treatment of demodecidosis. Methods Jatropha curcas.1 leaves were extracted with 80% ethanol by using heat reflux method. Facial sebum specimen from demodecidosis-infected patients were used to isolate and identify demodecidosis. Different concentration groups of Jatropha curcas.l leaves, 2% metronidazole control group and physiological saline control group were defined. The anti-demodecidosis experiment was performed in vitro. The pH value of different concentrations of Jatropha curcas.l leaves extract was determined. Skin irritation test and acute skin toxicity test were carried out in healthy rabbits, and Jatropha curcas.1 leaves and 75% ethanol served as experiment and control groups, respectivelyl Results The mite-killing time was （1.55-4-0.67） min with 50 mg/ml Jatropha curcas.l leaves extract, （1.61-4-0.67） min with 25 mg/ml Jatropha curcas.l leaves extract, （2.47-4-0.80） min with 12 mg/ml Jatropha curcas.l leaves extract, and （1.20-4-0.48） min with 2% metronidazole. There was no significant different between 50, 25 mg/ml Jatropha curcas.l leaves extract groups and 2% metronidazole group （P 〉 0.05）. The pH value was 6.07＋0.73 of 50 mg/ml Jatropha curcas.l leaves extract, 6.27-1-0.82 of 25 mg/ml Jatropha curcas.l leaves extract and 6.35-4-0.83 of 12 mg/ml Jatropha curcas.1 leaves extract. Score for irritation to normal and wounded rabbit skin was both 0, and acute toxicity test showed no significant toxicity. Conclusions Jatropha curcas.l leaves extract shows a remarkable activity to demodecidosis with skin safety in vitro.