小麦和绿豆根形态及细胞结构对喜马拉雅紫茉莉根水浸提液的化感作用响应

Allelopathy Effects of Aquatic Extracts from Mirabilis himalaica Roots on Morphology and Cell Structure of Wheat and Mung Bean Root

为了探究喜马拉雅紫茉莉(Mirabilis himalaica)根部水浸提液的化感作用,以小麦和绿豆作为供试材料进行了研究,结果表明这两种植物在经过喜马拉雅紫茉莉根部的水浸提液处理8 d后,细胞和组织形态结构受到了明显的化感抑制作用影响。利用透射电子显微镜和扫描电子显微镜分别观察和分析两种作物在水浸提液处理后的根系形态和细胞结构,结果发现小麦和绿豆的根尖结构已不完整,部分表皮细胞脱落,细胞间的空隙加大,组织间出现了断层和空腔,细胞间紧密排列的结构也被打乱,有内含物渗出;细胞膜萎缩,逐渐发生了质壁分离,细胞质中液泡变大,细胞核出现了消融现象,一些主要的细胞器如内质网、线粒体、高尔基体等结构遭受了破坏或解体,数量减少,植物生命活动受到了严重的抑制。以上结果反映了喜马拉雅紫茉莉根部的水浸提液对两种常见作物都具有较强的化感抑制作用。

To figure out the allelopathy effects of aquatic extracts from Mirabilis himalaica roots,we used wheat and mung bean as target plants for this study. Results showed that the cell and tissue morphologies of the two target plants were in significantly allelopathy and inhibitory effects after the treatment by the aquatic extracts from M. himalaica roots for 8 d. We observed and analyzed the root morphology and cell structure of the two crops treated with the aquatic extracts using transmission electron microscopy and scanning electron microscopy. The root tip structure in wheat and mung bean were incomplete,part of the epidermis cell felled off,the space between the cells turned larger,interstitial faulting and cavity appeared,and tightly structure between cells were disrupted. In the cell of wheat and mung bean root,membrane shrank, plasmolysis appeared,vacuoles in the cytoplasm turned bigger,nucleus ablated,and the structure of main organelles such as endoplasmic reticulum,mitochondria and Golgi apparatus varied or collapsed and depleted in numbers ;i.e.,plant life severely restrained. These results imply that the aquatic extracts from M. himalaica roots presented the strong allelopathic inhibition on two common crops.