高效液相色谱-三重四极杆质谱法快速测定真菌气溶胶示踪物

Rapid determination of fungal aerosol tracers using high performance liquid chromatography-tandem mass spectrometry

为实现真菌气溶胶示踪物的准确、快速、高灵敏测定,通过高效液相色谱和三重四极杆质谱联用,优化相关分析参数,建立了对阿糖醇和甘露醇这2种目标有机示踪物无需衍生化处理即可进行直接检测的快速分析方法.结果显示,在流动相氨水的浓度0.0001%、流速选择0.4 mL·min^(-1)、柱温箱温度45℃、负离子模式条件下,2种目标有机示踪物的线性范围是0.01—1 mg·L^(-1)(R^2>0.99),检测限分别达到5.6μg·L^(-1)(阿糖醇)、9.4μg·L^(-1)(甘露醇).该方法检测限低、灵敏度高、样品回收率高、重现性好,并具有较好的实用性.

An accurate,fast and sensitive analytical method for fungi aerosol tracers including both arabitol and mannitol has been developed using high performance liquid chromatography（HPLC）-tandem mass spectrometry（MS/MS） without any derivatization process,which is able to greatly reduce sample preparation time.The optimum analytical condition has been established as below：ammonium hydroxide（ω：0.0001%） as mobile phase with a flow rate of 0.4 m L·min^（-1）,oven temperature of 45 ℃,and negative ion electrospray ionization.Under the abovementioned analytical condition,the linear range of the two organic tracers is 0.01—1 mg·L^（-1）（R^2 0.99） and limits of detection（LOD） are 5.6 μg·L^（-1）（arabitol） and 9.4 μg·L^（-1）（mannitol）,respectively.After validation and application to the real aerosol samples,this new method has been proved to be a reliable and cost-effective alternative technique for analysis of both arabitol and mannitol.