双酚A对秀丽隐杆线虫的急性毒性作用研究

Study on Acute Toxicity of Bisphenol A to Caenorhabditis Elegans

双酚A(BPA)是一种普遍存在的内分泌干扰物,可以对人体健康产生不良影响。日常生活中,人们接触的BPA浓度较低,而低浓度下BPA毒性效应却并不明确。为了了解低浓度下BPA的毒性效应,我们以秀丽隐杆线虫为模式生物,以低浓度的BPA为研究对象,开展了急性毒性研究。研究结果表明BPA对秀丽线虫的LC50为440.16 mg/L,而低浓度的BPA对秀丽线虫的运动行为指标(头部摆动频率、身体弯曲频率)均具有显著的抑制作用,对头部摆动频率影响最为敏感,最低作用浓度为1μmol/L。低浓度的BPA还会影响秀丽线虫的生长发育和子代数目,与对照组相比,BPA浓度为100μmol/L时,体长和子代数目分别降低6.05%和15.24%。当BPA达到0.01μmol/L时,能够显著抑制秀丽线虫的寿命并且提高ROS水平,引起氧化应激的伤害,加速秀丽线虫的衰老,缩短其寿命。总之,低浓度的BPA暴露可以影响秀丽线虫的运动、生长发育、生殖及寿命。

Abstract： Bisphenol A （BPA） is a ubiquitous endocrine disrupter that can have side effect on human health. In daily life, people are exposed to low concentration of BPA, while the effect of BPA toxicity is not clear at low concentrations. In order to understand the toxic effects of BPA at low concentration, we used Caenorhabditis elegans （C. elegans） as a model organism to study the acute toxicity of low concentration of BPA. The results showed that the LCs0 of BPA was 440.16 mg/L for C. elegans, while the low concentration of BPA had significant inhibitory effect on the movement behavior indexes （including head thrashing frequency and body bending frequency） of C. elegans. The most sensitive index was the head thrashing frequency and the lowest concentration was 1 Imaol/L. Low concentration of BPA also affected the growth and development of C. elegans as well as the number of progenies. Compared with the control group, BPA at the concentration of 100 ~rnol/L reduced body length and progeny by 6.05% and 15.24%, respectively. When the concentration of BPA reached 0.01 pmol/L, it could significantly inhibit the life span of C. elegans and increase the level of ROS, causing oxidative stress injury, accelerating the senescence of C. elegans and shortening its life span. In conclusion, low concentration of BPA exposure could affect the movement, growth, reproduction and lon^eviW of C. eleeans.