不同钙片产品体外快速持钙活性与原子吸收法测定含钙量的比较研究

Comparison of Calcium Content in Different Calcium Products by Rapid Calcium-Holding Activity in Vitro and Atomic Absorption Spectrometry

酪蛋白磷酸肽(CPP)是从牛乳酪蛋白中水解分离所得到的一种生物活性肽,具有很强的促钙吸收活性。本实验考察CPP抑制磷酸钙/碳酸钙沉淀的产生,来表征CPP的持钙效果。对市售三种钙片体外持钙活性进行了体外快速演示实验和利用原子吸收法测定含钙量的比较和研究,结果表明含CPP的钙片具有较明显的持钙优势,阻止磷酸钙/碳酸钙聚沉时间可达30 min以上。在相同钙片重量和相同钙含量的情况下,加有CPP的钙片在水中溶解后的沉淀明显少于另外两种钙片产品,上清液加入碳酸钠溶液中,沉淀明显较多于另外两种钙片,表明了CPP具有明显延长持钙时间、有效抑制碳酸钙沉淀生成的作用。这一研究发现为市场上钙片产品补钙效果提供了有理有据的科学支撑,对保健品行业健康稳健的发展具有重大指导意义。

Casein phosphopeptide（CPP）, hydrolyzed and separated from milk casein, was a bioactive peptide and had a strong calcium absorption activity. The inhibition of calcium phosphate/calcium carbonate precipitation was investigated by CPP to characterize the calcium-holding effect of CPP in the present study. The calcium-holding activity of three commercial calcium tablets was investigated by rapid demonstration experiments in vitro, and the comparison of calcium content was determined by atomic absorption spectrometry. The results showed that the CPP-containing calcium tablets had an obvious advantage of calcium-holding and prevented calcium phosphate/calcium carbonate from precipitating for more than 30 minutes. In addition, the precipitation of CPP-containing calcium tablets was significantly less than the other two calcium products under the conditions of same calcium tablets content and calcium content while greatly more than the other two calcium tablets after treated with sodium carbonate solution, indicating that the CPP-containing calcium tablets had a significant effect on prolonging time calcium-holding and inhibiting the formation of calcium carbonate precipitation. The results of this study provided a reasonable scientific support for the calcium supplement effect of commercial calcium products and had a great guiding significance to the stable development of the health care industry.